Single cell gene expression profiling of NK cell specific Regnase-1 conditional knock-out mice

NK 细胞特异性 Regnase-1 条件敲除小鼠的单细胞基因表达谱

• 批准号: 20K22777
• 负责人: SUN XIN
• 金额: $ 1.83万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-09-11 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20K22777/
• 关键词: Bioinformatics; System Immunology; single cell RNAseq; NK cells; Regnase-1

In this research, 10x genomics single cell RNA-seq was applied into the splenic NK cells and TIL(tumor infiltrating)-NK cells both from Reg-1fl/fl(control) and Reg1ΔNK(Reg conditional KO mice). We found that: 1. Deletion of Regnase-1 decreased number of NK cells. 2. Reg1ΔNK produced high amount of cytotoxic proteins, such as IFN-g;, perforin and granzymes and showed anti-tumor activity both in invo and in vitro.

本研究将10x基因组学单细胞RNA-seq应用于regg -1fl/fl（对照）和Reg1ΔNK（regg条件型KO小鼠）的脾脏NK细胞和TIL（肿瘤浸润）NK细胞。我们发现：1。regase -1的缺失使NK细胞数量减少。2. Reg1ΔNK产生大量的细胞毒性蛋白，如IFN-g；在体内和体外均表现出抗肿瘤活性。