Novel Rat Model and Intervention for Elucidating the Molecular Mechanism in Chronic Postsurgical Pain

阐明慢性术后疼痛分子机制的新型大鼠模型和干预措施

• 批准号: 21H03023
• 负责人: 山内 正憲
• 金额: $ 10.98万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21H03023/
• 关键词: 遷延性術後痛; ラット疼痛モデル; 高周波パルス法神経ブロック; 次世代DNAシークエンサー; トランスクリプトーム解析

東北大学麻酔科では知覚過敏が60日間持続するラット下肢CPSPモデルの開発に成功した．本研究ではまずこのラット下肢CPSPモデルにおける脊髄やDRGでの遺伝子発現特性をゲノム網羅的に検討する．またラットCPSPモデルの脊髄・末梢神経への高周波パルス(PRF: Pulsed Radiofrequency)療法の影響を疼痛行動，電気生理学的および分子生物学的に解析する．PRFは神経組織へのダメージが少なく，痛みを中枢へ伝えるC繊維とAδ繊維だけに，数週間に限って変化を起こす．本研究ではラットCPSPモデルにPRF療法を行い，機序や効果を検討する．最終的には肺手術患者でPRF療法の臨床導入の可能性を探る．2022年度までに多くの試行錯誤を経て，自家製のPRF機器のセットアップは完了した．基盤実験で適切なPRFの発生と42℃を超えない熱産生も確認できている．この機器は通電量が自由に可変可能で，われわれのプロジェクトに最適である．

Tohoku University's Department of Anesthesiology has learned about allergy and successfully managed the lower limb CPSP program for 60 days. In this study, the characteristics of the lower limb CPSP lower limb CPSP DRG DRG were collected and the characteristics of the lower limbs were collected. The impact of PRF (Pulsed Radiofrequency) therapy on pain action, analysis of molecular biology of electrophysiology and molecular biology of CPSP. PRF is a sacred tissue that is less and less, and the center of pain is the center of the disease. This study is based on the results of CPSP PRF therapy and its effectiveness. Finally, we explore the possibility of clinical introduction of PRF therapy in lung surgery patients. In 2022, many trials and errors were carried out, and the self-made PRF machine was completed. The base plate is suitable for the PRF and the heat generated at 42℃ is super high and the heat generation is confirmed. The power of the machine is free and possible, and the power of the machine is the most suitable.