Identification and characterization of photosynthetic and translational modulators involved in chloroplast acclimation to low temperature (A04)

参与叶绿体低温适应的光合和翻译调节剂的鉴定和表征（A04）

• 批准号: 317544399
• 金额: --
• 依托单位: Max-Planck-Institut für molekulare Pflanzenphysiologie (MPI-MP)
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/317544399?language=en
• 关键词: Identification; characterization; photosynthetic; translational; modulators

We previously identified several plastid-encoded non-essential genes as candidate modulators of cold acclimation. Now, we elucidate the regulation of these genes and their physiological role in cold acclimation of photosynthesis and translation. Furthermore, we will analyse the cold response of translation by whole-cell ribosome profiling to obtain a quantitative, codon-resolved picture of this process and to identify additional translationally regulated modulators and integrators of cold acclimation. Moreover, we will unravel the compositional dynamics of the plastid translation machinery during cold acclimation to reveal regulatory functions of ribosomal proteins and ribosome-associated factors.

我们以前确定了几个质体编码的非必需基因作为候选调节冷驯化。现对这些基因的调控及其在光合和翻译冷驯化中的生理作用进行综述。此外，我们将通过全细胞核糖体分析来分析翻译的冷响应，以获得该过程的定量、密码子解析的图像，并确定冷驯化的额外的附加的调节性调节剂和整合剂。此外，我们将解开冷驯化过程中质体翻译机制的组成动态，以揭示核糖体蛋白和核糖体相关因子的调节功能。