Mechanisms and components of GUN1/GLK signalling and its relevance for acclimation (C05)

叶绿体蛋白基因组解偶联1(GUN1)和核转录因子Golden-like 1和2(GLK1和2)在去甲氟拉松或林可霉素处理过程中，通过下调Lhcb1标记基因的表达，在相同的逆行信号通路中发挥作用。我们在之前的工作中发现GUN1和GLK在冷适应中起着作用。我们的项目包括：(I)GUN1-GLK在寒冷中的生理特性，(Ii)通过遗传筛选鉴定新的GUN1-GLK信号成分，(Iii)剖析GUN1介导的GLK转录调控的顺式调控元件，以及(Iv)GLK靶标的特征。

The plastid protein GENOMES UNCOUPLED 1 (GUN1) and the nuclear transcription factors GOLDEN-LIKE1 and 2 (GLK1 and 2) operate in the same retrograde signalling pathway defined by de-repressed LHCB1 marker gene expression during treatment with norflurazon or lincomycin. We found in preceding work that GUN1 and GLK play a role in acclimation to cold. Our project includes: (i) Physiological characterisation of GUN1-GLK functions in the cold, (ii) Identification of novel of GUN1-GLK signalling components by genetic screens, (iii) Dissection of cis-regulatory elements of GUN1-mediated GLK transcriptional regulation, and (iv) Characterization of GLK targets.