权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

習慣・目的志向行動の脳内機構の構成論的解明

建设性地阐明习惯和目标导向行为的大脑机制

基本信息

批准号：
21H03492
负责人：
船水章大
金额：
$ 10.98万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2021
资助国家：
日本
起止时间：
2021-04-01 至 2026-03-31
项目状态：
未结题

项目摘要

ヒトや動物は，ひとつの状況に対して複数の行動戦略を用意し，その戦略を使い分ける．本研究は，戦略切り替えの神経回路基盤を，神経科学と機械学習を統合した学際的手法で解明する．具体的には，マウスの行動実験と行動モデル化，マウスの神経活動計測と操作を用いる．次年度は，まず，初年度に構築したマウスの行動課題を継続して実施した．行動課題は，音周波数の遷移確率pを操作することで，習慣行動・目的志向行動を分離できる．本研究では，p=0.8に設定した切り替え条件とp=0.2の連続条件を，別々の野生型マウスで実施した．次に，合計12匹のマウス (4匹の連続条件，8匹の切り替え条件) で，大脳新皮質の前頭葉・頭頂葉・聴覚野の神経活動を大規模計測した．これまでに，2万細胞以上の神経活動を計測した．予備的な解析の結果，前頭葉だけでなく，頭頂葉や聴覚野でも，習慣行動・目的志向行動の両行動に相関する神経活動を発見した．今後，詳細な神経活動解析を実施する．次に，行動課題時のマウスで，神経活動を抑制する実験系を整えた．上述の神経活動解析の結果，前頭葉が習慣行動・目的志向行動に寄与する可能性がある．そこで，GtACR2を発現したアデノ随伴ウイルスを前頭葉に注入し，行動課題時に前頭葉を光抑制する実験系を開発した．また，前年度に引き続き，ChR2を抑制性細胞に発現した遺伝子改変マウスで，背側皮質の各領野を抑制する実験も実施した．結果を解析中である．

The purpose of this study is to help God to return to the roadbed, and to understand the methods used in the science of science, mechanics, and science. The specific activities of the animals, the activities of the animals, the operations of the activities, and the operation of the activities of the animals have been verified, and the operation of the activities of the gods has been used in the next year. In the beginning of the year, you will be responsible for the operation of the project, the number of sound waves, the transfer rate of the number of sound cycles, the transfer rate of the target, and the separation of the target actions. in this study, packs 0.8 sets the switching conditions, packs 0.2, connection conditions, and applies to wild-type machines. A total of 12 dogs (4 connection conditions, 8 cut-off conditions) were used to analyze the activities of large-scale models (over 20,000 cells). The results of the analysis of the equipment, the results of the analysis of the results, the results of the analysis of the results of the equipment, the results of the analysis of the results of the equipment, the results of the analysis of the results of the equipment, the results of the analysis of the equipment, and the results of the analysis of the equipment. In the future, the activities of the gods will be analyzed, and the activities of the gods will be reached. on the second occasion, the activities of the gods will inhibit the activities of the gods, and the results of the above-mentioned analysis of the activities of the gods, the gods GtACR2 has been shown to be in the process of activating the photoinhibition system during the operation, and the previous year has been introduced, and the ChR2 suppression cell has been used to improve the performance of the system, and the results have been analyzed.