Adipose tissue-specific determinants of the vascularization capacity of isolated microvascular fragments for tissue engineering

实验研究表明，分离的脂肪组织来源的微血管片段是组织工程中有效的血管生成单位。这些碎片种植到支架上后，会迅速发育成新的微血管网络，并在植入后与宿主组织的血管系统相互连接。在我们的研究项目中，我们将研究脂肪组织特异性决定因素如何影响这一过程，这些决定因素对未来的临床应用具有重要意义。为此，我们将在体外和体内的小鼠模型中，分析来自皮下和内脏脂肪组织的小鼠微血管片段的血管形成能力取决于脂肪供体的性别、营养状况和年龄。在额外的原理验证实验中，我们将首次从人类脂肪组织中分离和表征微血管片段，并将它们用于植入支架的预血管形成。这样，就有可能获得成功地将基于微血管片段的血管形成策略转化为临床实践所必需的知识。

Experimental studies indicate that isolated adipose tissue-derived microvascular fragments are effective vascularization units for tissue engineering. After their seeding onto scaffolds, these fragments rapidly develop into new microvascular networks, which interconnect with the vascular system of the host tissue after implantation. In our research project, we will investigate how this process is influenced by adipose tissue-specific determinants, which are of fundamental importance for the future clinical application. For this purpose, we will analyze the vascularization capacity of murine microvascular fragments from subcutaneous and visceral adipose tissue in dependence on the gender, nutritional status and age of the fat donors in vitro and in an in vivo mouse model. In additional proof-of-principle experiments we will isolate and characterize for the first time microvascular fragments from human adipose tissue and use them for the prevascularization of implanted scaffolds. In this way, it will be possible to obtain essential knowledge for the successful translation of microvascular fragment-based vascularization strategies into clinical practice.