Adipose tissue-specific determinants of the vascularization capacity of isolated microvascular fragments for tissue engineering

用于组织工程的分离微血管碎片的血管化能力的脂肪组织特异性决定因素

• 批准号: 318765517
• 负责人: Professor Dr. Matthias W. Laschke, Ph.D.
• 金额: --
• 依托单位: Institut für Klinisch-Experimentelle Chirurgie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/318765517?language=en
• 关键词: Adipose; tissue; specific; determinants; vascularization

Experimental studies indicate that isolated adipose tissue-derived microvascular fragments are effective vascularization units for tissue engineering. After their seeding onto scaffolds, these fragments rapidly develop into new microvascular networks, which interconnect with the vascular system of the host tissue after implantation. In our research project, we will investigate how this process is influenced by adipose tissue-specific determinants, which are of fundamental importance for the future clinical application. For this purpose, we will analyze the vascularization capacity of murine microvascular fragments from subcutaneous and visceral adipose tissue in dependence on the gender, nutritional status and age of the fat donors in vitro and in an in vivo mouse model. In additional proof-of-principle experiments we will isolate and characterize for the first time microvascular fragments from human adipose tissue and use them for the prevascularization of implanted scaffolds. In this way, it will be possible to obtain essential knowledge for the successful translation of microvascular fragment-based vascularization strategies into clinical practice.

实验研究表明，分离的脂肪组织来源的微血管片段是组织工程中有效的血管化单元。在它们接种到支架上之后，这些片段迅速发育成新的微血管网络，这些微血管网络在植入后与宿主组织的血管系统互连。在我们的研究项目中，我们将研究这一过程是如何受到脂肪组织特异性决定因素的影响，这对未来的临床应用至关重要。为此目的，我们将在体外和体内小鼠模型中，根据脂肪供体的性别、营养状况和年龄，分析来自皮下和内脏脂肪组织的小鼠微血管片段的血管化能力。在另外的原理验证实验中，我们将首次从人类脂肪组织中分离和表征微血管片段，并将其用于植入支架的预血管化。通过这种方式，将有可能获得必要的知识，以成功地将基于微血管片段的血管化策略转化为临床实践。