Development of amino acid-based cationic lipid nanoparticles specialized for cytosolic gene delivery

以赖氨酸型阳离子脂质K3C16为主要成分，研究了脂质纳米颗粒（LNP）组成对pDNA转染和细胞毒性的影响。对不同类型和比例的辅助脂质进行了挑战，发现1.5mol% PEG2000和10mol% DOPE的转染效率最高，与Lipofectamin3000相比，没有显著的细胞毒性。此外，还成功实现了利用SHM制备LNP的微流控方法，该方法可以制备出粒径在100 nm左右的微量LNP，并且具有比传统体块法更好的单分散性。在精氨酸型脂质的研究中，揭示了脂质结构对T细胞活化的影响，并发表了一篇学术论文。

The effect of lipid nanoparticle (LNP) composition on pDNA transfection and cytotoxicity had been investigated using lysine type cationic lipids K3C16 as the main component. Various types and ratios of helper lipids were challenged and it was found that 1.5mol% PEG2000 and 10mol% DOPE endowed the best transfection efficiency among all the tested groups without significant cytotoxicity in comparison to Lipofectamin3000. In addition, microfluidic method using SHM for LNP preparation had been successfully achieved, which allowed trace amount fabrication with fine tuned size around 100 nm and a better monodispersity than conventional bulk method.In the study of arginine type lipids, the lipid structure effect on T cell activation was disclosed and one academic paper was published.