Durchflusszytometer

该应用程序用于高端多色流式细胞仪，以取代最初安装于1995年的老化的三色机。近年来，高度多重和多色分析在流式细胞术中变得越来越普遍。这是由于荧光染料和功能染料的可用性增加，用于流式细胞术的颜色比以前多得多，以及各种波长的固体激光的可用性增加，现在可以用来激发这些新的染料和染料。新的细胞仪将使我们能够对通常细胞数量有限的样本进行高度多元化的实验，通过结合新的标记、新的荧光蛋白组合以及功能染色的可能性来识别新的细胞群体。细胞仪将被放置在一个新建立的专门研究荧光技术的核心设施中。这将使来自Uniklinik的研究人员能够接触到新机器，并从该设施经验丰富的成员那里获得关于实验规划和适当使用仪器的支持。

The application is for a high-end, multicolor flow cytometer to replace an aging 3-color machine originally installed in 1995. Highly multiplexed and multicolor analyses have become increasingly prevalent in recent years in flow cytometry. This is due to the increased availabilty of fluorescent dyes and functional stains for flow cytometry in many more colors than were previously available, as well as to an increased availability of solid state lasers of a variety of wavelengths which can now be used to excite these new dyes and stains. The new cytometer would enable us to carry out highly multiplexed experiments on samples which are often limited in cell number, allowing the identification of new populations of cells through the possibility of combining new markers, new combinations of fluorescent proteins, and also functional stains. The cytometer would be placed in a newly established core facility specialized in fluorescence technologies. This will allow researchers from across the Uniklinik to have access to the new machine, as well as receive support with regard to experimental planning and proper use of the instrumentation from the experienced members of the facility.