Multienzymatic Cascade in a 2-Phase System - Process integration

生物催化作为一种可替代的化工生产工艺，在化学工业中发挥着越来越重要的作用。在这种情况下，多酶级联反应有可能满足复杂合成路线的需求，具有高选择性和高纯度的产物，并且不需要在两者之间进行产物分离。特别是体外系统具有高时空产出的潜力，尽管它们不那么复杂，相对简单的数学描述，因此很容易优化。在两相系统中成功建立多酶级联反应后，计划通过工艺整合加强。为此，级联的反应步骤与萃取步骤以及随后在一个装置中分离两相相结合。因此，介绍了第一台三相（l/l/s）反应萃取离心机。这种独特的装置同时也是第一台酶反应萃取离心机。这个高度创新的项目旨在促进平台技术的发展，以组合通常单独执行的流程步骤。因此，可以更好地理解生物催化中的复杂综合过程。该系统将被描述为数学和建模。最后给出了基于系统各个步骤的整个流程模型，为流程优化提供了有价值的帮助。

Biocatalysis becomes more and more important as an alternative production process in chemical industry. In this context multi-enzyme cascade reactions have the potential to meet the demand for complex synthesis routes with high selectivity and purity of the products and no need for product isolation in between. Especially in-vitro systems have a potential for high space-time yields, although they are less complex, comparatively simple to describe mathematically and therefor good to optimize. Following the successful establishment of a multi-enzyme cascade reaction in the 2-phase system, intensification by process integration is planned. For this purpose, a reaction step of the cascade is combined with the extraction step and the subsequent separation of the two phases in one apparatus. Therefore, the first 3-phase (l/l/s) reactive extraction centrifuge is introduced. This unique apparatus is at the same time the first enzymatic reactive extraction centrifuge.This highly innovative project aims to contribute the development of a platform technology to combine process steps, which are usually individually performed. Thus, a better understanding of complex integrated processes in biocatalysis can be achieved. The system will be described mathematical and modelled. In the end a whole process model will be given, based on the individual system steps, to give a valuable assistance for process optimization.