Enzymatic conversion of kelp biomass for biofuels and health beneficial products.

将海带生物质酶促转化为生物燃料和有益健康的产品。

• 批准号: 20H02776
• 负责人: FOX BRIAN
• 金额: $ 11.23万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20H02776/
• 关键词: enzymatic hydrolysis; alginate; laminarin; fukoidan; health benefit; Kelp hydrolysis; Alginate; Laminarin; kelp hydrolysis; GH55 enzyme; PL enzymes; Kelp hydrolysate; kelp-degrading enzymes; Enzyme kelp hydrolysis; Chemical composition; Gen

To achieve an effective enzyme combination for enzymatic hydrolysis of laminarin and alginate in kelp, a glycoside hydrolase family 55 enzyme (SactELam55A) and two polysaccharide enzyme families (PLs), seven PL17 and two PL18 were successfully prepared using a recombinant enzyme production method. The reaction optima of temperature and pH of these enzymes were individually determined for combinatorial enzyme hydrolysis. Additionally, one reported PL7 enzyme was gene synthesized to produce monomer from alginate polymer in combination with PL17 and PL18 alginate depolymerizing enzymes.

为了实现海带中海带多糖和海藻酸盐的有效酶组合，采用重组酶法制备了一个糖苷水解酶家族55(SactELam55A)和两个多糖酶家族(PLs)、七个PL17和两个PL18。分别确定了三种酶组合酶解的最适温度和pH。此外，一种已报道的PL7酶被基因合成，与PL17和PL18海藻酸解聚酶一起由海藻酸聚合物产生单体。