ショットガンメタゲノミクスによるシロアリ腸内微生物群集の進化動態の解明

使用鸟枪法宏基因组学阐明白蚁肠道微生物群落的进化动态

• 批准号: 20J10912
• 负责人: Arora Jigyasa
• 金额: $ 1.09万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-24 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20J10912/
• 关键词: metagenomics; termites; microbes

I successfully defended my PhD thesis in March, 2022. The thesis chapter one orthe first AIM has been accepted in the Microbiome journal (impact factor 14.65). I examined the evolution of termite gut microbial functions across 150 million years of evolution.I also finished the analysis, figures, and manuscript writing for the second AIM of the project, in which I analyzed the gut microbial composition in 202 termite samples across termite evolutionary history. The manuscript for this project is almost ready for submission to a high-impact factor journal.Finally, the last AIM of the project was analyzed as part of the third chapter of the thesis. This chapter involved examining microbial gene family evolution using phylogenetic methods to investigate the role of Horizonal Gene Transfer and multiple acquisitions from the environment on metabolic genes. The top ten microbial gene families were examined in the thesis chapter, which is now being scaled up to the remaining hundred gene families. The scripts have been standardized, and the manuscript will be ready in the next few months.

我在2022年3月成功地答辩了我的博士论文。论文第一章或第一个AIM已被微生物组杂志接受（影响因子14.65）。我研究了白蚁肠道微生物功能在1.5亿年进化过程中的演变。我还完成了该项目第二个AIM的分析、数字和手稿撰写，其中我分析了白蚁进化历史中202个白蚁样本的肠道微生物组成。最后，作为论文第三章的一部分，对该项目的最后一个目标进行了分析。本章涉及使用系统发育方法研究微生物基因家族进化，以研究水平基因转移和从环境中多次获取对代谢基因的作用。在论文章节中研究了前十个微生物基因家族，现在正在扩大到剩下的一百个基因家族。剧本已经标准化，手稿将在未来几个月内完成。

项目成果

The functional evolution of termite gut microbiota.

• DOI: 10.1186/s40168-022-01258-3
• 发表时间: 2022-05-27
• 期刊: MICROBIOME
• 影响因子: 15.5
• 作者: Arora, Jigyasa;Kinjo, Yukihiro;Sobotnik, Jan;Bucek, Ales;Clitheroe, Crystal;Stiblik, Petr;Roisin, Yves;Zifcakova, Lucia;Park, Yung Chul;Kim, Ki Yoon;Sillam-Dusses, David;Herve, Vincent;Lo, Nathan;Tokuda, Gaku;Brune, Andreas;Bourguignon, Thomas
• 通讯作者: Bourguignon, Thomas

Gut microbiome evolution across 150 million years of termite evolution

白蚁 1.5 亿年进化过程中的肠道微生物组进化

• 发表时间: 2020
• 作者: 野口洋平;上出寛子;田中文英;SunKyoung Kim;白石伝助;Arora Jigyasa
• 通讯作者: Arora Jigyasa

150 million years of evolution of gut microbiome in termites

白蚁肠道微生物组1.5亿年的进化

• 发表时间: 2020
• 作者: Sato Kenichiro;Ihara Ryoko;Suzuki Kazushi;Niimi Yoshiki;Toda Tatsushi;Jimenez‐Maggiora Gustavo;Langford Oliver;Donohue Michael C.;Raman Rema;Aisen Paul S.;Sperling Reisa A.;Iwata Atsushi;Iwatsubo Takeshi;Arora Jigyasa
• 通讯作者: Arora Jigyasa