Development of novel transfection system using DNA gel

在最近的研究中，人们研究了DNA自组装结构与聚合物组成的液-液相分离体系之间的相互作用。据观察，DNA结构可以被这些隔间选择性地占据，导致模仿人造细胞的自组装以及核酸和细胞器的相互作用。基于这些发现，我开发了一种纯化自组装DNA结构的方法，并基于DNA折纸在液滴界面的积累创建了一种新的胶体类别。这使得胶体能够自组织成细胞状结构。在未来，我将探索通过DNA结节体膜转移细胞核的可能性，这可能会在DNA测序技术和用于核酸跨膜运输的合成细胞模型中得到应用。

In recent research, the interaction between self-assembled DNA structures and liquid-liquid phase-separated systems composed of polymers was investigated. It was observed that DNA structures can be selectively taken up by these compartments, leading to self-assembly that mimics artificial cells and the interaction of nucleic acids and organelles. Based on these findings, I developed a method for purifying self-assembled DNA structures and created a new category of colloidossomes based on the accumulation of DNA origami at the interface of droplets. This enabled the creation of colloidosomes that can self-organise into cellular like structures. In the future, I will explore the possibility of translocating nucleic through membranes of DNA coloidosomes, which might find application in DNA sequencing technology and on synthetic cell models for the transport of nucleic acids thourgh membranes.