Development of novel transfection system using DNA gel

使用 DNA 凝胶开发新型转染系统

• 批准号: 20J11059
• 负责人: MASUKAWA Marcos
• 金额: $ 1.09万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-24 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20J11059/
• 关键词: DNA origami; colloidosome; self-assembly; aqueous two-phase system; aqueous-aqueous emulsion; DNA hydrogel; purification; monodisperse; microfluidic

In recent research, the interaction between self-assembled DNA structures and liquid-liquid phase-separated systems composed of polymers was investigated. It was observed that DNA structures can be selectively taken up by these compartments, leading to self-assembly that mimics artificial cells and the interaction of nucleic acids and organelles. Based on these findings, I developed a method for purifying self-assembled DNA structures and created a new category of colloidossomes based on the accumulation of DNA origami at the interface of droplets. This enabled the creation of colloidosomes that can self-organise into cellular like structures. In the future, I will explore the possibility of translocating nucleic through membranes of DNA coloidosomes, which might find application in DNA sequencing technology and on synthetic cell models for the transport of nucleic acids thourgh membranes.

在最近的研究中，研究了自组装DNA结构与聚合物组成的液-液相分离体系之间的相互作用。观察到DNA结构可以选择性地被这些隔室占据，导致模仿人工细胞的自组装以及核酸和细胞器的相互作用。基于这些发现，我开发了一种纯化自组装DNA结构的方法，并基于DNA折纸在液滴界面上的积累创造了一种新的胶体质体。这使得胶体体能够自组织成类似细胞的结构。在未来，我将探索通过DNA胶体小体膜进行核易位的可能性，这可能会在DNA测序技术和核酸通过膜运输的合成细胞模型中得到应用。

项目成果

DNA structures inside aqueous-aqueous emulsions - assembly, purification and folding

水-水乳液内的 DNA 结构 - 组装、纯化和折叠

• 发表时间: 2020
• 作者: Marcos Masukawa;Masahiro Takinoue
• 通讯作者: Masahiro Takinoue

Purification of DNA nano/microstructures by aqueous two-phase system

水性两相系统纯化 DNA 纳米/微米结构

• 发表时间: 2021
• 作者: Marcos Masukawa;Fujio Yu;Yusuke Sato;Kanda Tsumoto;Kenichi Yosikawa;Masahiro Takinoue
• 通讯作者: Masahiro Takinoue

Purification of DNA nano/microstructures by aqueous-aqueous two-phase system fractionation

通过水-水两相系统分级纯化 DNA 纳米/微米结构

• 发表时间: 2021
• 作者: Marcos Masukawa;Fujio Yu ;Yusuke Sato ;Kanta Tsumoto;Kenichi Yoshikawa;Masahiro Takinoue
• 通讯作者: Masahiro Takinoue

DNA-polymer emulsions: self-assembly and purification of DNA structures

DNA-聚合物乳液：DNA 结构的自组装和纯化

• 发表时间: 2021
• 作者: Marcos Masukawa;Fujio Yu;Yusuke Sato;Kanta Tsumoto;Kenichi Yoshikawa;Masahiro Takinoue
• 通讯作者: Masahiro Takinoue

Purification and room temperature folding of DNA origami

DNA折纸的纯化和室温折叠

• 发表时间: 2020
• 作者: Marcos Masukawa;Kanta Tsumoto;Kenichi Yoshikawa;Masahiro Takinoue
• 通讯作者: Masahiro Takinoue