Preparation of anti-Arsenic based Antibodies and Development of ELISA for Arsenic Analysis in the Environment

抗砷抗体的制备及环境砷分析ELISA方法的开发

• 批准号: 32356415
• 负责人: Dr. Sathrugnan Karthikeyan, Ph.D.
• 金额: --
• 依托单位: Lehrstuhl für Analytische Chemie und Wasserchemie
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/32356415?language=en
• 关键词: Preparation; anti; Arsenic; based; Antibodies

Arsenic is commonly found throughout the environment in a wide variety of chemical species that vary in toxicity and mobility. Humans are exposed to a considerable amount of arsenic through drinking water and food and to some extent, through contaminated air depending on geographical areas. As a result of these health concerns, arsenic research has grown steadily in recent years in accord with the need to understand the occurrence, distribution, and mobilization of arsenic in the environment and to develop cost-effective treatment technologies.New instrumental techniques are being developed at a rapid rate in an effort to produce analytical methods with lower detection limits, shorter temporal resolution, and increased selectivity with the goal of enhancing our understanding of fate and transport of arsenic. In general, a field deployable instrument can be of better choice since it enables us to do real-time analysis. Immunoassay technology offers a new approach and has significant advantages over the traditional instrument-intensive methods of metals analysis. They are remarkably quick, easy, high throughput and feasible for field analysis. The aim of the present research is to prepare antibodies against arsenic species. This will be used in development of Enzyme-linked Immunosorbent Assay (ELISA) to allow easy and cheap analysis of these species in different samples, e.g. in water samples in Asia.

砷通常存在于环境中的各种化学物质中，毒性和流动性各不相同。人类通过饮用水和食物暴露于大量的砷，并在一定程度上通过受污染的空气，这取决于地理区域。由于这些健康问题，近年来，砷的研究稳步增长，这与了解砷在环境中的发生、分布和移动以及开发具有成本效益的处理技术的需要是雅阁的。并增加选择性，以提高我们对砷的命运和运输的理解。一般来说，一个现场部署的仪器可以是更好的选择，因为它使我们能够做实时分析。免疫分析技术提供了一种新的方法，与传统的仪器密集型金属分析方法相比具有显着的优势。它们非常快速，简单，高通量，并适用于现场分析。本研究的目的是制备针对砷物种的抗体。这将用于开发酶联免疫吸附测定法（ELISA），以方便和廉价地分析不同样品中的这些物种，例如亚洲的水样。