Preparation of anti-Arsenic based Antibodies and Development of ELISA for Arsenic Analysis in the Environment

砷通常存在于环境中各种各样的化学物质中，其毒性和流动性各不相同。人类通过饮用水和食物接触到相当数量的砷，在某种程度上通过受污染的空气接触到砷，这取决于地理区域。由于这些健康问题，砷研究近年来稳步发展，符合了解环境中砷的发生、分布和流动的需要，并开发具有成本效益的处理技术。新的仪器技术正在迅速发展，以产生更低的检出限、更短的时间分辨率和更高的选择性的分析方法，目的是加强我们对砷的命运和运输的理解。一般来说，现场可部署仪器是更好的选择，因为它使我们能够进行实时分析。与传统的仪器密集型金属分析方法相比，免疫分析技术提供了一种新的方法，具有显著的优势。它们具有快速、简便、高通量和可用于现场分析的特点。本研究的目的是制备抗砷的抗体。这将用于开发酶联免疫吸附试验（ELISA），以方便和廉价地分析不同样品中的这些物种，例如在亚洲的水样中。

Arsenic is commonly found throughout the environment in a wide variety of chemical species that vary in toxicity and mobility. Humans are exposed to a considerable amount of arsenic through drinking water and food and to some extent, through contaminated air depending on geographical areas. As a result of these health concerns, arsenic research has grown steadily in recent years in accord with the need to understand the occurrence, distribution, and mobilization of arsenic in the environment and to develop cost-effective treatment technologies.New instrumental techniques are being developed at a rapid rate in an effort to produce analytical methods with lower detection limits, shorter temporal resolution, and increased selectivity with the goal of enhancing our understanding of fate and transport of arsenic. In general, a field deployable instrument can be of better choice since it enables us to do real-time analysis. Immunoassay technology offers a new approach and has significant advantages over the traditional instrument-intensive methods of metals analysis. They are remarkably quick, easy, high throughput and feasible for field analysis. The aim of the present research is to prepare antibodies against arsenic species. This will be used in development of Enzyme-linked Immunosorbent Assay (ELISA) to allow easy and cheap analysis of these species in different samples, e.g. in water samples in Asia.