セレウス菌食中毒の重篤患者発生予防：調理食品での毒素産生影響要因の解明を中心に

预防严重蜡样芽孢杆菌食物中毒：重点阐明影响熟食产毒的因素

• 批准号: 20K02359
• 负责人: 河合 高生
• 金额: $ 2.66万
• 依托单位: Osaka Institute of Public Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20K02359/
• 关键词: セレウス菌; 嘔吐毒; セレウリド; 食中毒; LC-MS/MS; 調理食品

喫食後短時間に一過性の嘔吐を発症するセレウス菌嘔吐型食中毒では、まれに肝障害や脳症等の重篤な症状を呈し、死亡例が発生することもある。本研究では、セレウス菌食中毒による重篤患者発生リスクの低減を図るため、細菌学的および遺伝学的手法を用い、調理食品中でのセレウス菌嘔吐毒(セレウリド)の産生性を向上させる様々な要因を調べ、重篤患者が発生しうる危険度の高い食品や条件を解明すること、並びに、迅速診断用に患者材料からのセレウリド検出法を開発することを目的としている。これまでの結果より、例え食中毒由来株であっても菌株によって寒天培地上でのセレウリド産生性が異なることが判明している。このセレウリドの産生性の違いに、セレウリド合成酵素遺伝子群の塩基配列変化が関係しているかどうかを調べるため、由来が異なるセレウス菌保存株60株の中から、セレウリド産生性が異なる菌株を６段階のクラスに分け、それぞれのクラスから2株以上計18株を選定した。令和5年4月に実施予定とした次世代シーケンサーによるゲノム解析に向け、この18株のDNA抽出を行った。また、MALDI-TOF MSを使用したセレウリドの検出法に関して情報収集を行った。

Within a short period of time after eating, the symptoms of severe symptoms such as vomiting, liver damage and other severe symptoms, and death cases were reported. In this study, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, the patients with severe bacterial food poisoning, Quickly discontinue the use of patient materials. Please do not use the method to do this. The results showed that the cause of food poisoning caused by food poisoning was caused by a strain of food poisoning. In cold weather, it was found that the disease was caused by the disease. The subgroup of synthetic enzymes was sequenced, and the subgroup of synthetic enzymes was sequenced. the results showed that there were 60 strains of bacteria, 6 strains of biogenic strains and more than 18 strains of biosynthetic enzymes. In April 2005, Ling and April of 2005, the next generation of DNA was extracted from the next generation. In this case, you can use the MALDI-TOF MS file to set the data set in a row.