Apical cell growth and generation of cellular asymmetry in corynebacteria (P05)

棒状杆菌中顶端细胞的生长和细胞不对称性的产生（P05）

• 批准号: 326464754
• 金额: --
• 依托单位: Institut für Allgemeine Mikrobiologie
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/326464754?language=en
• 关键词: Apical; cell; growth; generation; cellular

Corynebacterium glutamicum is a model organism to study apical growth. The spatiotemporal organization of apical cell wall synthesis machineries and polar tethering of the chromosome are governed by the scaffold protein DivIVA. Corynebacteria have an unusually complex cell envelope termed mycoloyl-arabinogalactan-peptidoglycan (mAGP) complex. We will analyze the spatial organization of the mAGP synthesis by DivIVA. DivIVA was also identified as polar tether for the ParB–parS nucleoprotein complex. It remains unsolved how newly replicated ParB complexes are forming and how the segregation process of sister origins is initiated. We will address ParB complex formation and dynamics in the new funding period. Furthermore, we will unravel the function of a novel, DivIVA-localized DNA-repair mechanism. Our work will provide a comprehensive insight into the cell biology of an apically growing bacterium and contribute to the understanding of the intimate connection of apical growth on chromosome organization.

谷氨酸棒杆菌是研究根尖生长的模式生物。顶端细胞壁合成机制的时空组织和染色体的极性束缚由支架蛋白DivIVA控制。棒状杆菌具有异常复杂的细胞被膜，称为分枝菌酰-阿拉伯半乳聚糖-肽聚糖（mAGP）复合物。我们将通过DivIVA分析mAGP合成的空间组织。DivIVA也被鉴定为ParB-parS核蛋白复合物的极性系链。新复制的ParB复合物是如何形成的，以及姐妹起源的分离过程是如何开始的，这些问题仍然没有解决。我们将在新的资助期内解决ParB复合体的形成和动态。此外，我们将揭示一种新的DivIVA定位的DNA修复机制的功能。我们的工作将提供一个全面的了解顶端生长的细菌的细胞生物学，并有助于了解染色体组织顶端生长的密切联系。