大腸菌の　Ｔｒｋ／Ｋｔｒ／ＨＫＴ　トランスポーターの新規機能の解明

阐明大肠杆菌中 Trk/Ktr/HKT 转运蛋白的新功能

• 批准号: 19J10317
• 负责人: Ellen (2020)
• 金额: $ 1.22万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-04-25 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19J10317/
• 关键词: K+ transporter; Trk/Ktr/HKT

This proposed research aims to explore new feature of Trk/Ktr/HKT-type potassium ion (K+) transporter. We focused on to investigate the distinct feature of Escherichia coli Trk-type transporters. The K+ transporter knock-out mutants were successfully constructed to examine the sole activity of TrkG and TrkH, respectively. The uptake experiments showed a different characteristics between TrkG and TrkH activity, indicated that these two transporters are non-identical and possibly contribute to different physiological functions for survivability. The better understanding of Trk transporter in E. coli will give a new insight for other Trk/Ktr/HKT superfamily transporters that expressed not only in bacteria, but also in the economically important organisms, such as yeast and crop plants.

本研究旨在探索Trk/Ktr/HKT型钾离子(K+)转运蛋白的新特性。我们重点研究了大肠杆菌Trk型转运蛋白的显著特征。成功构建了K+转运蛋白敲除突变体，分别用于检测TrkG和TrkH的单一活性。摄取实验显示TrkG和TrkH活性具有不同的特征，表明这两种转运蛋白是不同的，可能参与了不同的生存生理功能。对Trk转运蛋白在大肠杆菌中的深入研究将为其他Trk/Ktr/HKT超家族转运蛋白的研究提供新的视角，这些转运蛋白不仅在细菌中表达，而且在酵母和农作物等具有重要经济意义的生物中也有表达。

项目成果

Kup-Driven Cs+ Uptake Promote Escherichia coli Growth under K+ Limited Condition

Kup驱动的Cs摄取促进大肠杆菌在有限K条件下的生长

• 发表时间: 2019
• 作者: Tatsuhiro Nagasaka;Hikaru Sotome;Tsuyoshi Kawai;Hiroshi Miyasaka;Ellen
• 通讯作者: Ellen