マイクロ流路デバイス中の流れと空間の制御による超高速イムノセンシングの実現

通过控制微流控装置中的流量和空间实现超高速免疫传感

• 批准号: 19J11009
• 负责人: Sathish Shivani
• 金额: $ 1.34万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-04-25 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19J11009/
• 关键词: microfluidic; biomarker detection; kinetic enhancements; protein immobilization; PMMA microfluidics; Point of care testing; Fluid handling

When a person is afflicted with a disease, quantities of biomarkers are elevated. This research is focused on developing sensitive and rapid microfluidic systems to detect a wide range of biomarkers. Here, kinetic enhancements in microfluidic bioassay systems were investigated using novel 3D glass devices. Real-time binding events between fluorescently-labelled receptor and ligand antibodies were analyzed. Combining experimental measurements with scaling analysis, two key control dimensionless parameters were proposed to achieve “rapid” and “sensitive” ligand detection: a local Peclet number and a kinetic Damkohler number. Finally, we summarized our observations by providing design rules to support the development of fast and sensitive microfluidic bioassay systems.

当一个人患有某种疾病时，生物标志物的数量就会升高。这项研究的重点是开发敏感和快速的微流体系统，以检测各种生物标志物。在这里，动力学增强微流控生物测定系统进行了研究，使用新型的3D玻璃设备。荧光标记的受体和配体抗体之间的实时结合事件进行了分析。结合实验测量与标度分析，提出了两个关键的控制无量纲参数，以实现“快速”和“灵敏”的配体检测：本地Peclet数和动力学Damkohler数。最后，我们总结了我们的观察，提供设计规则，以支持快速和灵敏的微流控生物检测系统的发展。

项目成果

Surface-based Microfluidic Systems for Enhanced Biomarker Detection

• DOI: 10.15102/1394.00001803
• 发表时间: 2021-04
• 期刊: Solid Fuel Chemistry
• 影响因子: 0.7
• 作者: S. Sathish

Development of cheap point-of-care devices for early and rapid disease diagnosis

开发用于早期和快速疾病诊断的廉价床旁设备

• 发表时间: 2019
• 作者: Sathish;Shivani
• 通讯作者: Shivani

“Integrated prototype for point-of-care diagnosis of Chlamydia trachomatis infections

“沙眼衣原体感染即时诊断的集成原型

• 发表时间: 2019
• 作者: Sathish;Shivani
• 通讯作者: Shivani