マイクロ流路デバイス中の流れと空間の制御による超高速イムノセンシングの実現

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When a person is afflicted with a disease, quantities of biomarkers are elevated. This research is focused on developing sensitive and rapid microfluidic systems to detect a wide range of biomarkers. Here, kinetic enhancements in microfluidic bioassay systems were investigated using novel 3D glass devices. Real-time binding events between fluorescently-labelled receptor and ligand antibodies were analyzed. Combining experimental measurements with scaling analysis, two key control dimensionless parameters were proposed to achieve “rapid” and “sensitive” ligand detection: a local Peclet number and a kinetic Damkohler number. Finally, we summarized our observations by providing design rules to support the development of fast and sensitive microfluidic bioassay systems.