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Hippocampal Functional Organization in Fear Memory

恐惧记忆中的海马功能组织

基本信息

批准号：
19J20225
负责人：
荒井翔子
金额：
$ 1.98万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2019
资助国家：
日本
起止时间：
2019-04-25 至 2022-03-31
项目状态：
已结题

项目摘要

It is well known that the entorhinal cortex (EC) is one of the main sources of inputs to the hippocampus necessary for the formation of episodic memory. However, how multimodal factors in episodic memory are processed in the EC is not well understood. In the present study, we investigated individual EC sub-regional contributions in episodic memory to determine a possible functional dissociation of EC sub-regions. We fear conditioned animals on a plus maze with two different types of stimuli - spatial or object, which is processed in MEC and LEC, while optogenetically inhibiting MEC/LEC. I finished behavioral experiments and statistically analyzed behavioral and histological data.In weak foot-shock condition (0.3mA), LEC inhibited animals (n=6) showed significantly lower avoidance toward shocked object than control animals (n=7; p < 0.005). Avoidance toward the shocked arm of MEC inhibited animals (n=8) was also comparatively lower than controls (n=7; p = 0.06). In strong foot-shock condition (1mA), LEC inhibited (n=6) as well as control (n=8) animals showed over 90% avoidance toward shock object, however, LEC inhibited animals avoided not only the shocked but also no-shocked object significantly longer than controls (p < 0.05). In place fear conditioning, MEC inhibited animals (n=7) tended to avoid the no-shocked arm, which is adjacent to the shocked arm, longer than control animals (n=4; p = 0.07). These results suggest that EC sub-regions process information about specific place/object as well as associate the information with fear memory.

众所周知，内嗅皮层（EC）是情景记忆形成所需的海马输入的主要来源之一。然而，情景记忆中的多模态因素在 EC 中是如何处理的尚不清楚。在本研究中，我们研究了个体 EC 子区域对情景记忆的贡献，以确定 EC 子区域可能的功能分离。我们担心条件动物在十字迷宫中受到两种不同类型的刺激——空间刺激或物体刺激，这些刺激在MEC和LEC中进行处理，同时光遗传学抑制MEC/LEC。我完成了行为实验，并对行为和组织学数据进行了统计分析。在弱足部电击条件下（0.3mA），LEC 抑制动物（n=6）对电击物体的回避程度显着低于对照动物（n=7；p < 0.005）。 MEC 抑制动物 (n = 8) 对电击手臂的回避也相对低于对照组 (n = 7; p = 0.06)。在强烈的足部电击条件下 (1mA)，LEC 抑制动物 (n=6) 以及对照动物 (n=8) 表现出超过 90% 的对电击物体的回避，然而，LEC 抑制动物不仅回避电击物体而且回避未电击物体的时间明显长于对照组 (p < 0.05)。在适当的恐惧调节中，MEC 抑制动物 (n=7) 倾向于避开与电击手臂相邻的未电击手臂，其长度比对照动物 (n=4；p = 0.07) 更长。这些结果表明，EC 子区域处理有关特定地点/物体的信息，并将这些信息与恐惧记忆联系起来。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Hippocampal Functional Organization in Contextual Fear Memory Task

情景恐惧记忆任务中的海马功能组织

DOI：
发表时间：
2019
期刊：
影响因子：
0
作者：
Shoko Arai;Krzysztof A. Sypniewski and Constantine Pavlides;Shoko Arai and Constantine Pavlides
通讯作者：
Shoko Arai and Constantine Pavlides

The role of the medial and lateral entorhinal cortex in memory.

内侧和外侧内嗅皮层在记忆中的作用。