Membrane protein organization studied at the single molecule level using liquid-phase electron microscope (C07)

使用液相电子显微镜在单分子水平上研究膜蛋白组织（C07）

• 批准号: 340464713
• 金额: --
• 依托单位: Leibniz-Institut für Neue Materialien gGmbH (INM)
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/340464713?language=en
• 关键词: Membrane; protein; organization; studied; single

ORAI1 and HER2 proteins are both known to control the dynamics of the actin cytoskeleton, the main component of the cellular structure responsible for cell migration playing a pivotal role for instance in cancer metastasis. However, the underlying mechanisms, as well as their interdependencies are still poorly understood. Using liquid phase electron microscopy, we study the biophysical principles of how a cell or-ganizes these membrane proteins in specific locations in a functional configuration in relation to actin such to regulate function.

已知ORAI1和HER2蛋白都控制肌动蛋白细胞骨架的动力学，肌动蛋白细胞骨架是负责细胞迁移的细胞结构的主要组分，例如在癌症转移中起关键作用。然而，人们对其潜在机制及其相互依赖性仍然知之甚少。利用液相电子显微镜，我们研究了细胞如何在特定位置以与肌动蛋白相关的功能构型组织这些膜蛋白以调节功能的生物物理原理。