TRR 51: Ecology, Physiology and Molecular Biology of the Roseobacter Clade: Towards a Systems Biology Understanding of a Globally Important Clade of Marine Bacteria

TRR 51：玫瑰杆菌分支的生态学、生理学和分子生物学：对全球重要的海洋细菌分支的系统生物学理解

• 批准号: 34509606
• 金额: --
• 依托单位: Carl von Ossietzky Universität Oldenburg
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/34509606?language=en
• 关键词: TRR; 51; Ecology; Physiology; Molecular

The overarching goal of this Transregional Collaborative Research Centre (CRC) TRR51 “Roseobacter” is a comprehensive understanding of the evolutionary and adaptational success of the Roseobacter group in marine ecosystems. Members of this group are globally distributed and often represent prominent components of the bacterial communities or carry out specific processes important in the given ecological context. Members of the Roseobacter group encompass very different and versatile physiologies. The mission of TRR51 is to investigate the Roseobacter group with respect to their evolution and phylogeny and the genome structure and content regarding its versatile physiology and ecology. These investigations cover environmental and biogeography studies on the community level as well as detailed investigations on the physiology of individual organisms and systems biology studies of the two model organisms, Dinoroseobacter shibae and Phaeobacter inhibens. Investigations in the third application phase will focus on interactions of these organisms, mainly with two cosmopolitan coastal algae Thalassiosira rotula and Prorocentrum minimum. Further, interactions with and the significance of phages will become another focus. The investigations are still structured in Project Area A, focusing on the ecology and evolution, Project Area B focusing on genetics and physiology and Project Area C focusing on systems biology of the two model organisms. The environmental studies, mainly home in project area A, will complete the analyses of samples and data collected during two cruises with Research Vessel Sonne in the Pacific Ocean investigating a longitudinal transect around 180°E between the southern polar frontal water masses at 52°S and the subarctic waters in the Bering Sea at 59°N. Work included sampling the water column and surface sediment for bacterioplankton activity and community composition, metagenomics, -transcriptomics, -proteomics and geometabolomics. These investigations aim at a comprehensive and synoptic assessment of these data together with those of previous cruises to the Southern and Atlantic Ocean in order to arrive at a synoptic and predictive model of the functional role of pelagic and benthic lineages of the Roseobacter group in the global oceans. Studies with the model organisms D. shibae and P. inhibens, home in all three project areas, will focus on interactions with the two model algae, T. rotula and P. minimum. The genome of both algae will be sequenced. Topics include the impact on the endo- and exometabolome, the role of specific exometabolites on growth of the algae, the role of quorum sensing, oxygen tension, iron and temperature in interactions, growth control and metabolite exchange, and significance of extrachromosomal elements (ECR). The different effects of direct physical interactions and interactions mediated via exchange of dissolved metabolites and how they impact the joint exometabolome of both partners will be examined

这个跨区域合作研究中心（CRC）TRR 51“Roseback”的总体目标是全面了解Roseback群在海洋生态系统中的进化和适应成功。该组的成员分布于全球，通常代表细菌群落的主要组成部分，或在特定的生态环境中进行重要的特定过程。玫瑰花组的成员包括非常不同和多才多艺的生理。TRR 51的使命是研究蔷薇属植物群的进化和生殖，以及与其多功能生理学和生态学有关的基因组结构和内容。这些调查包括在群落层面进行的环境和地理研究，以及对个别生物的生理学进行的详细调查，以及对两种模式生物-甲藻和拟甲藻的系统生物学研究。第三个应用阶段的调查将侧重于这些生物的相互作用，主要是与两种世界性的沿海藻类圆海链藻和最小原甲藻的相互作用。此外，与互联网的互动和意义将成为另一个焦点。调查仍在项目区A中进行，重点是生态学和进化，项目区B重点是遗传学和生理学，项目区C重点是两种模式生物的系统生物学。环境研究主要在项目区A进行，将完成对研究船索内在太平洋进行的两次航行中收集的样本和数据的分析，调查南纬52度的南极锋面水团和北纬59度的白令海亚北极沃茨之间的一条180度左右的纵向断面。工作包括采样的水柱和表层沉积物的浮游细菌活动和社会组成，宏基因组学，转录组学，蛋白质组学和geometabolomics。这些调查的目的是对这些数据以及以前在南大洋和大西洋进行的考察的数据进行全面的天气评估，以便建立一个全球海洋中Roseberry组中上层和底栖谱系功能作用的天气预测模型。以模式生物D.在所有三个项目区域都有家的shiobis和P. lubbens，将专注于与两种模式藻类T。rotula和P. minimum。这两种藻类的基因组将被测序。主题包括对内和外代谢组的影响，特定外代谢组对藻类生长的作用，群体感应的作用，氧张力，铁和温度的相互作用，生长控制和代谢物交换，以及染色体外元素（ECR）的意义。将检查直接物理相互作用和通过溶解代谢物交换介导的相互作用的不同影响，以及它们如何影响双方的联合外代谢组