Analysis of EPLIN function in early embryonic development and mouse ES cells

EPLIN在早期胚胎发育和小鼠ES细胞中的功能分析

• 批准号: 351978311
• 负责人: Dr. Ivan Bedzhov
• 金额: --
• 依托单位: Forschungsgruppe Embryonic Self-Organization
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/351978311?language=en
• 关键词: Analysis; EPLIN; function; early; embryonic

E-cadherin mediated cell adhesion is essential for embryonic development and is specifically required for morula compaction and blastocyst formation. These morphogenic processes coincide with the establishment of the first cell types in the developing mouse embryo. E-cadherin connection to the cytoskeleton via EPLIN (Epithelial Protein Lost In Neoplasm) is extensively studied in vitro, mainly in cancer cell lines, however EPLIN function in vivo is not characterized. Using the mouse as a model system, we want to elucidate a potential role of EPLIN in early embryogenesis, maintenance and differentiation of mouse embryonic stem cells, as well as to generate tools for analyzing EPLIN function in adult tissues.

e -钙粘蛋白介导的细胞粘附对胚胎发育至关重要，是桑葚胚压实和囊胚形成所必需的。这些形态形成过程与发育中的小鼠胚胎中第一批细胞类型的建立一致。E-cadherin通过EPLIN （Epithelial Protein Lost In Neoplasm，上皮蛋白丢失）与细胞骨架的连接在体外被广泛研究，主要是在癌细胞系中，然而EPLIN在体内的功能尚未被表征。以小鼠为模型系统，我们希望阐明EPLIN在小鼠胚胎干细胞早期胚胎发生、维持和分化中的潜在作用，并为分析EPLIN在成年组织中的功能提供工具。