iPS cell generation from jaw periosteum: is the resulting osteogenic capacity more efficient?

口腔颌面外科领域迫切需要新的有效疗法，以使特别是大的骨缺损再生。组织工程是最有前途的治疗方法。颌骨骨膜细胞（JPC）在咀嚼过程中承受高机械负荷的独特特化，使其成为口腔颌面外科细胞治疗的有吸引力的候选者。在我们合作项目的第一个资助期，我们首次从颌骨骨膜细胞中产生了诱导多能干细胞（iPS），并成功地发表了这项工作。我们建立了一个完全无异源的方案，并使用自我复制的RNA构建体，以进行无足迹的细胞重编程。下一步，我们从这些iPS细胞中产生了诱导的间充质基质细胞（iMSCs），这些细胞在二维培养中表现出很强的矿化潜力。我们继续开发iMSC种植的3D结构和测试细胞功能，（特别是分化潜力）和生物相容性的研究。通过本项目的实现，我们为骨组织工程开辟了新的干细胞来源，并为基于iPS细胞的治疗建立了临床适用的方法。

New and effective therapies are urgently needed in the field of oral and maxillofacial surgery to enable regeneration of in particular large bone defects. Tissue engineering strategies represent the most promising therapeutic option.The unique specialization of jaw periosteal cells (JPCs) withstanding high mechanical load during mastication, makes them to attractive candidates for cell-based therapies for oral and maxillofacial surgeries.In the first funding period of our joint project, we generated for the first time induced pluripotent stem (iPS) cells from jaw periosteal cells and successfully published this work. We established a completely xeno-free protocol and used a self-replicating RNA construct in order to perform a footprint-free cell reprogramming. In the next step, we generated induced mesenchymal stromal cells (iMSCs) from these iPS cells, which in turn showed strong mineralization potential in 2D culture.In view of future clinical application, we move forward to develop iMSC-seeded 3D constructs and test cell functions (particularly the differentiation potential) and biocompatibility of constructs in vitro and in vivo by using an immunodeficient mouse model.By the realization of this project, we open up a new stem cell source for bone tissue engineering and establish a clinically applicable approach for iPS cell-based therapies.