The modular function of the y-secretase in the generation of intermediate Aß species (D03)

y-分泌酶在中间 Aà 物种生成中的模块化功能 (D03)

• 批准号: 35817922
• 金额: --
• 依托单位: Department of Pharmacology and Therapeutics
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/35817922?language=en
• 关键词: modular; function; secretase; generation; intermediate

Processing of the amyloid precursor protein by the ß-secretase and the γ-secretase module leads to the generation of amyloid ß-peptides and a soluble intracellular C-terminal domain, which is involved in transcriptional regulation. The goal of this project is stepwise to reconstitute the enzyme/substrate complex and to structurally analyze the interaction of multiple transmembrane segments by pulsed electron–electron double resonance spectroscopy based on a GxxxG motif which was found to mediate helix-helix interactions within subunits of the γ-secretase module.

ß-分泌酶和γ-分泌酶模块对淀粉样蛋白前体蛋白的加工导致淀粉样蛋白ß-肽和参与转录调节的可溶性细胞内c -末端结构域的产生。本项目的目标是逐步重建酶/底物复合物，并基于GxxxG基序，通过脉冲电子-电子双共振光谱分析多个跨膜片段的相互作用，该基序被发现在γ-分泌酶模块的亚基内介导螺旋-螺旋相互作用。