トキソプラズマ原虫に対する宿主免疫系におけるIRGB6の役割の解明

阐明 IRGB6 在宿主免疫系统中针对弓形虫的作用

• 批准号: 18K05970
• 负责人: 李 英愛
• 金额: $ 2.83万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-04-01 至 2020-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18K05970/
• 关键词: Irgb6; Toxoplasma gondii; Ubiquitination; Pphospholipid; IFN誘導性GTPase; トキソプラズマ; 寄生胞; 人畜共通感染症; インターフェロン

We characterized the role of IFN-γ-inducible immunity-related GTPase Irgb6 in the cell-autonomous response against Toxoplasma gondii (T. gondii), which involves vacuole ubiquitination and breakdown. We show that Irgb6 is capable of binding a specific phospholipid on the parasitophorous vacuole (PV)membrane. Furthermore, the absence of Irgb6 causes reduced targeting of other effector IRG proteins to the PV. This suggests that Irgb6 has a role as a pioneer in the process by which multiple IRG proteins access the PV.

我们研究了IFN-γ诱导的免疫相关的GT3/Irgb 6在抗弓形虫（Toxoplasma gondii，T. gondii），其涉及液泡泛素化和分解。我们表明，Irgb 6是能够结合特定的磷脂的寄生虫空泡（PV）膜。此外，Irgb 6的缺乏导致其他效应IRG蛋白对PV的靶向降低。这表明Irgb 6在多个IRG蛋白进入PV的过程中具有先锋作用。

项目成果

An effector IRG is a critical factor mediating interferon-g-induced ubiquitin decoration of Toxoplasma gondii parasitophorous vacuoles

效应子 IRG 是介导干扰素 g 诱导的弓形虫寄生空泡泛素修饰的关键因素

• 发表时间: 2019
• 作者: Bando Hironori;Lee Youngae;Sakaguchi Naoya;Pradipta Ariel;Ma Ji Su;Tanaka Shun;Cai Yihong;Liu Jianfa;Shen Jilong;Nishikawa Yoshifumi;Sasai Miwa;Yamamoto Masahiro;Youngae Lee
• 通讯作者: Youngae Lee