Neuronal regulation of glutamate homeostasis in addictive behavior

成瘾行为中谷氨酸稳态的神经元调节

• 批准号: 364631096
• 负责人: Theresa Rimmele, Ph.D.
• 金额: --
• 依托单位: F.M. Kirby Neurobiology Center
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/364631096?language=en
• 关键词: Neuronal; regulation; glutamate; homeostasis; addictive

Glutamate homeostasis−the control of glutamate in and around synapses, has been shown to be critical in regulating addictive behavior. It has been assumed that astrocytes play the dominant role in determining glutamate homeostasis. Using a conditional knockout in the glutamate transporter GLT-1, the major glutamate transporter in the brain, we have found that inactivating GLT-1 in neurons has a significant impact on the effects of the psychostimulant amphetamine. These results suggest that neuronal control of glutamate homeostasis is a critical factor in psychostimulant addiction. The study aims to characterize the described phenomenon in more detail and investigate the underlying mechanisms using behavioral, molecular biology and fluorescence imaging approaches.

谷氨酸稳态-突触内和周围谷氨酸的控制，已被证明是调节成瘾行为的关键。 据推测，星形胶质细胞在确定谷氨酸稳态中起主导作用。 使用谷氨酸转运蛋白GLT-1（大脑中主要的谷氨酸转运蛋白）的条件性敲除，我们发现神经元中的GLT-1失活对精神兴奋剂安非他明的作用有显著影响。这些结果表明，神经元对谷氨酸稳态的控制是精神兴奋剂成瘾的一个关键因素。该研究旨在更详细地描述所描述的现象，并使用行为、分子生物学和荧光成像方法研究其潜在机制。