Towards integrating the AHL transcription factors into a plant growth gene regulatory network

将 AHL 转录因子整合到植物生长基因调控网络中

• 批准号: 19F19781
• 负责人: 杉本 慶子
• 金额: $ 1.41万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-11-08 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19781/
• 关键词: AHL; SOB3; petiole; PIF; transcription; growth; plants; photomorphogenesis

My work during the past funding period has provided new insights into the mechanisms by which the AHL transcription factors regulate transcription and growth in Arabidopsis. Together with my collaborators at Washington State University, I finished generating data for and writing a manuscript that describes how AHLs regulate gene expression and suppress hypocotyl growth in seedlings grown in short days. We recently resubmitted this manuscript to BMC Plant Biology, where it has been provisionally accepted. In this manuscript, we describe how AHLs affect the expression of different sets of genes at different times throughout the course of a short day. For example, we showed that SOB3 and/or other AHLs repress the expression of the PIF-activated, growth-promoting genes XTR7 and CDF5 at ZT9 and ZT24, but not at ZT4. On the other hand, we found that SOB3 and/or other AHLs activate the expression of the growth-repressing genes HY5, BBX22, RGA, and PRR9 specifically at ZT4. However, we unexpectedly also found that SOB3 exhibits similar patterns of binding to target genes at ZT4, ZT9, and ZT24. Thus, our data from this study collectively suggest that AHLs affects gene expression in a time-point specific manner irrespective of changes in binding to DNA throughout short days. Apart from work related to the BMC Plant Biology manuscript, I have also recently generated data suggesting that AHLs affect PIF activity at the protein level, i.e. they can reduce PIF-mediated activation of target genes without affecting PIF protein levels.

在过去的资助期间，我的工作为AHL转录因子调节拟南芥转录和生长的机制提供了新的见解。与我在华盛顿州立大学的合作者一起，我完成了数据的生成并撰写了一份手稿，描述了ahl如何调节基因表达并抑制短时间内生长的幼苗的下胚轴生长。我们最近将这篇文章重新提交给BMC Plant Biology，暂被接受。在这篇论文中，我们描述了ahl如何在短时间内影响不同基因组的表达。例如，我们发现SOB3和/或其他ahl抑制pif激活的生长促进基因XTR7和CDF5在ZT9和ZT24的表达，而不是在ZT4。另一方面，我们发现SOB3和/或其他ahl特异性激活生长抑制基因HY5、BBX22、RGA和PRR9在ZT4的表达。然而，出乎意料的是，我们还发现SOB3在ZT4、ZT9和ZT24位点与靶基因的结合模式相似。因此，本研究的数据共同表明，ahl以特定时间点的方式影响基因表达，而与短时间内与DNA结合的变化无关。除了与BMC Plant Biology手稿相关的工作外，我最近还生成了一些数据，表明ahl在蛋白质水平上影响PIF活性，即它们可以降低PIF介导的靶基因的激活，而不影响PIF蛋白水平。

项目成果

Molecular pathways regulating elongation of aerial plant organs: a focus on light, the circadian clock, and temperature

• DOI: 10.1111/tpj.14996
• 发表时间: 2020-10-18
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Favero, David S.;Lambolez, Alice;Sugimoto, Keiko
• 通讯作者: Sugimoto, Keiko

Cell Function Research Team

细胞功能研究团队

University of Potsdam(ドイツ)

波茨坦大学（德国）

AT-hook transcription factors promote photomorphogenesis by antagonizing the PHYTOCHROME INTERACTING FACTORs

AT-hook 转录因子通过拮抗 PHYTOCHROME 相互作用因子来促进光形态发生

• 发表时间: 2021
• 作者: Favero David S.;Kawamura Ayako;Shibata Michitaro;Takebayashi Arika;Jung Jae-Hoon;Suzuki Takamasa;Jaeger Katja E.;Ishida Takashi;Iwase Akira;Wigge Philip A.;Neff Michael M.;Sugimoto Keiko;Favero David S.;David S. Favero;David S. Favero
• 通讯作者: David S. Favero

The SUMO E3 Ligase SIZ1 Negatively Regulates Shoot Regeneration

• DOI: 10.1104/pp.20.00626
• 发表时间: 2020-09-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Coleman, Duncan;Kawamura, Ayako;Sugimoto, Keiko
• 通讯作者: Sugimoto, Keiko