Mitochondrial haplotypes and phenotypes in laying hens

线粒体参与了几个器官的关键过程，因此，在母鸡的有效磷（P）利用和肌醇代谢的mtDNA单倍型和mt基因表达的深入表征是至关重要的。已知肌醇通过迄今未知的与自噬和线粒体自噬相关的机制来调节线粒体功能。在拟议的项目中，我们将获得整个线粒体基因组单倍型的两个不同品种的蛋鸡在实验中获得的另一个项目在拟议的研究单位。这种方法确定了在产蛋鸡遗传选择过程中影响关键酶活性的潜在突变。为了检测不同单倍型之间的mt基因表达差异，将进行组织和发育特异性定量（q）PCR实验。我们结合联合收割机我们的数据与研究单位的其他项目中获得的基因组分析，以找到依赖于核背景的相互作用位点的协会。此外，线粒体DNA单倍型与代谢组学特征的分子信息相关，特别是在另一个项目中评估的作为线粒体功能指标的酰基肉毒碱。因此，我们将获得全面的了解线粒体在不同的植酸和磷利用和肌醇代谢母鸡的功能重要性。

Mitochondria are involved in several organismic key processes; hence the in-depth characterization of mtDNA haplotypes and mt gene expression in hens with efficient phosphorus (P) utilization and myo-inositol metabolism is of utmost importance. Myo-inositol is known to modulate mitochondrial functionality by so far unknown mechanisms related to autophagy and mitophagy. In the proposed project we will obtain whole mitochondrial genome haplotypes for two distinct strains of laying hens obtained in experiments of another project within the proposed Research Unit. This approach determines potential mutations that arose during the genetic selection process in laying hens affecting key enzyme activities. To test for mt gene expression differences among distinct haplotypes, tissue and developmental-specific quantitative (q) PCR experiments will be performed. We combine our data with those of genome analyses obtained in other projects of the Research Unit to find associations of interacting loci depending on the nuclear background. Further, mtDNA haplotypes are associated with the molecular information regarding metabolomics profiles, especially with acylcarnitines as indicators of mitochondrial function, assessed in another project. Thus, we will gain comprehensive insights into the functional importance of mitochondria in hens varying in phytate and P utilization and myo-inositol metabolism.