Quantitative fluorescence microscopy for the analysis of Wnt pathway interactions and dynamics (A06)

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In this collaborative project the quantification of Wnt ligand interactions will be extended to all ten FZD receptors, thereby gaining important, unresolved information on the native binding selectivity of a Wnt and its potential to dictate Wnt signaling specificity. FFS approaches (e.g., axial line-scanning FCS) will be used to quantify ligand-receptor and receptor-receptor interactions involved in signalosome formation. By super-resolution single-molecule localization microscopy imaging (SMLM) the stoichiometry and dynamics of Wnt receptor/co-receptor clusters and signalosome formation at the plasma membrane will be visualized. The project relies on both novel technologies and interdisciplinary collaborations within the consortium.