Protein–protein interactions at the synapse monitored by quantitative protein cross-linking (A10)

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State-of-the-art mass spectrometry (MS) makes possible the identification and quantification of cellular proteins, including the detailed analysis of post-translational modifications. MS has recently been combined with chemical cross-linking of proteins, with the purpose of identifying protein–protein contact sites. I now plan to extend cross-linking mass spectrometry to the investigation of protein interactions from native material. I will investigate isolated nerve terminals (synaptosomes) in resting and stimulated (K+/Ca2+ stimulation) states.