Protein–protein interactions at the synapse monitored by quantitative protein cross-linking (A10)

通过定量蛋白质交联监测突触处的蛋白质-蛋白质相互作用 (A10)

• 批准号: 386932925
• 金额: --
• 依托单位: Institut für Klinische Chemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/386932925?language=en
• 关键词: Protein; protein; interactions; synapse; monitored

State-of-the-art mass spectrometry (MS) makes possible the identification and quantification of cellular proteins, including the detailed analysis of post-translational modifications. MS has recently been combined with chemical cross-linking of proteins, with the purpose of identifying protein–protein contact sites. I now plan to extend cross-linking mass spectrometry to the investigation of protein interactions from native material. I will investigate isolated nerve terminals (synaptosomes) in resting and stimulated (K+/Ca2+ stimulation) states.

最先进的质谱（MS）技术使得细胞蛋白质的鉴定和定量成为可能，包括翻译后修饰的详细分析。MS最近已与蛋白质的化学交联结合，目的是鉴定蛋白质-蛋白质接触位点。我现在计划将交联质谱法扩展到研究天然物质中的蛋白质相互作用。我将研究孤立的神经末梢（突触体）在休息和刺激（K+/Ca 2+刺激）状态。