Molecular and functional analysis of the mechanism driving cytoplasmic streaming in Arabidopsis thaliana

细胞质流动对于分配细胞内化合物或对于它们在细胞中的战略定位是重要的。在植物中，细胞质流由肌球蛋白控制。三种类型的原则进行了讨论：动员的每一个细胞化合物通过特定的相互作用与肌球蛋白，动员的几个细胞器/RNP和comobilization的其他人的协会和搅拌的液相由一些细胞器/RNP。为了解决这个问题，我们表达了融合到特定锚在拟南芥突变体（mya 1 mya 2 xi-k），表现出几乎没有细胞质流。表达肌球蛋白-锚蛋白的突变肌球蛋白系，但不表达携带YFP-YFP而不是锚的阴性对照的系，显示出生长拯救。我们建议使用这些线来研究不同的细胞器/RNP拯救细胞质流的能力。此外，我们将通过研究细胞形态发生表型的拯救、肌动蛋白细胞骨架组织的拯救和突变体和拯救系中根毛中ROP 2信号传导来评估不同细胞器/RNP运动的生物学意义。

Cytoplasmic streaming is important to distribute intracellular compounds or for their strategic positioning in the cell. In plants, cytoplasmic streaming is governed by myosins. Three types of principles have been discussed: the mobilization of each cellular compound through specific interactions with myosins, the mobilization of few organelles/RNPs and comobilization of others by association and stirring of the liquid phase by some organelles/RNPs. To address this, we have expressed myosins fused to specific anchors in an Arabidopsis mutant (mya1 mya2 xi-k) that exhibits virtually no cytoplasmic streaming. Mutant myosin lines expressing myosin-anchor proteins but not lines expressing the negative control carrying an YFP-YFP instead of an anchor show growth rescue. We propose to use these lines to study the ability of different organelles/RNPs to rescue cytoplasmic streaming. In addition we will assess the biological significance of the movement of different organelles/RNPs by studying the rescue of the cell morphogenesis phenotypes, the rescue of the organization of the actin cytoskeleton and ROP2 signaling in root hairs in mutant and rescued lines.