Role of SUMOylation of Satb2 in the regulation of ES cell differentiation, gene expression and higher-order chromatin structure (P04)

Satb2 SUMO化在 ES 细胞分化、基因表达和高阶染色质结构调控中的作用 (P04)

• 批准号: 39236241
• 金额: --
• 依托单位: Max-Planck-Institut für Immunbiologie und Epigenetik
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/39236241?language=en
• 关键词: Role; SUMOylation; Satb2; regulation; ES

This project addresses the mechanisms by which the functional specificity of Satb2, a regulator of higher-order chromatin structure, can be altered by SUMO modification. We have previously shown that Satb2 plays important roles in the regulation of pluripotency of embryonic stem (ES) cells and in the higher-order chromatin structure of the immunoglobulin heavy chain gene locus in B cells. Recently, we found that Satb2 is SUMOylated during differentiation of ES cells by the novel SUMO E3 ligase Znf451. In the next funding period we will examine how Znf451-mediated SUMOylation of Satb2 alters the function of Satb2 in regulating ES cell pluripotency.

该项目致力于研究通过 SUMO 修饰改变 Satb2（高阶染色质结构调节因子）功能特异性的机制。我们之前已经表明，Satb2 在胚胎干（ES）细胞多能性的调节以及 B 细胞中免疫球蛋白重链基因座的高级染色质结构中发挥重要作用。最近，我们发现 Satb2 在 ES 细胞分化过程中被新型 SUMO E3 连接酶 Znf451 修饰。在下一个资助期内，我们将研究 Znf451 介导的 Satb2 SUMO 化如何改变 Satb2 调节 ES 细胞多能性的功能。