Hybrid Quadrupol Massenspektrometer

点击翻译按钮获取中文摘要

Mass-spectrometry (MS) based quantitative proteomics is a powerful technology used to study tissue, cellular and sub-cellular proteomes as well as protein post-translational modifications (PTMs). This technology has revolutionized the quantification of protein levels and modifications for in many biological fields, amongst them chronobiology. The circadian clock is an endogenous timing system that regulates in a daily manner physiological functions and behavior. The circadian clock exerts its control by rhythmically modulating gene expression and protein function. While circadian transcription regulation is widely studied the understanding of circadian dynamics in protein function is in its infancy. My work in the last years has pioneered the use of MS based quantitative proteomics to study circadian protein function by means of protein abundance, cellular relocalization and PTMs. This technology has become instrumental in the circadian field to investigate how global proteomes/PTMs are temporally regulated to thus comprehensively understand how circadian clocks regulate physiology and behavior. This is a prerequisite to further investigate metabolic, behavioral and cognitive disorders associated with circadian asynchrony, a condition frequently associated with modern life styles. Therefore, the acquisition of this state of the art instrumentation will tremendously boost chronobiology research as well as benefit and strengthen the proteomics community at the LMU.