Molecular mechanism of blue-light signal transduction in photosynthetic bacteria

光合细菌蓝光信号转导的分子机制

• 批准号: 05454015
• 负责人: TAKAMIYA Ken-ichiro
• 金额: $ 3.52万
• 依托单位: Tokyo Institute of Technology (TIT)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454015/
• 关键词: Photosynthetic bacteria; Blue-light effect; Rhodobacter sphaeroides; puf-operon; Trans-factor; Bacteriochlorophyll; Leucine-zipper motif; Two-componet regulatory system; puf-オペロン; ロイシン・ジッパーモチーフ; two-component system; リン酸化; ゲルシフトアセイ

1.A transcription factor (SPB) which regulates the expression of puf-operon in Rhodobacter sphaeroides was detected by the gel-shift assay and cloned after purification.2.SPB had molecular mass of 11.5 kDa and contained a lecine-zipper motif in the N-terminal region.3.SPB was constitutively expressed and phosphorylation/dephosphorylation of SPB regulated the expression of the puf-operon of the bacterium. (phosphorylated SPB repressed the expression of the puf-operon)4.A mutant in which SPB was disrupted exhibited higher pigment content and growth rate under low-light photosynthesis than the parent, whereas the growth rate by respiration was not altered.5.The results mentioned above suggested that SPB regulated the expression of the puf-operon as a repressor.6.DNA sequenccing of the flanking region of SPB revealed that there were a two=component regulatory system upstream of SPB and three ORFs downstream of SPB.

1.用凝胶转移法检测了球形红杆菌puf操纵子表达的转录因子（SPB），并对其进行了纯化和克隆。SPB的分子量为11.5 kDa，在n端含有一个卵磷脂拉链基序。SPB组成型表达，SPB的磷酸化/去磷酸化调节puf操纵子的表达。3 .磷酸化的SPB抑制puf操纵子的表达。SPB被破坏的突变体在弱光条件下的色素含量和生长速度高于亲本，而呼吸作用下的生长速度没有改变。上述结果表明，SPB作为抑制因子调控puf-operon的表达。SPB侧翼区DNA测序结果显示，SPB上游有一个双组分调控系统，下游有三个orf。

项目成果

Mizoguchi, H.: "Nucleotide sequence and transcriptional analysis of the flanking region of the gene (spb) for the trans-acting factor that controls light-mediated expression of the puf operon in Rhodobacter sphaeroides." Plant Cell Physiol.38 (in press).

Mizoguchi, H.：“反式作用因子基因 (spb) 侧翼区域的核苷酸序列和转录分析，该因子控制球形红杆菌中 puf 操纵子的光介导表达。”

Yun,C.-H.,et al.: "Deletion of the gene encoding cytochrome b562 from Rhodobacter sphaeroides." FEMS Microbiol.Lett.120. 105-110 (1994)

Yun,C.-H.,et al.：“从球形红杆菌中删除编码细胞色素 b562 的基因。”

Takamiya,K.: "Some properties and occurrence of cytochrome c-552 in the aerobic photosynthetic bacterium Roseobacter denitrificans." Arch.Microbiol.159. 51-56 (1993)

Takamiya,K.：“需氧光合细菌脱氮玫瑰杆菌中细胞色素 c-552 的一些特性和出现。”

Nishimura,K.: "Expression of the puf operon in an aerobic photosynthetic bacterium,Roseobacter denitrificans." Plant Cell Physiol.37. 153-159 (1996)

Nishimura,K.：“puf操纵子在需氧光合细菌脱氮玫瑰杆菌中的表达。”

Shimada,H.: "A transcription factor with a leucine-zipper motif involved in light-dependent inhibition of expression of the puf operon in the photosynthetic bacterium Rhodobacter sphaeroides." Plant Cell Physiol.37. 515-522 (1996)

Shimada,H.：“一种具有亮氨酸拉链基序的转录因子，参与光合细菌球形红杆菌中 puf 操纵子表达的光依赖性抑制。”