Studies on fine structure and function of rice photosynthetic apparatus

利用电子显微镜和SDS-PAGE对水稻叶绿体的相互转化进行了研究。从电子显微镜观察，从叶绿体到原生质体的脱脂过程是绿色化从原生质体到叶绿体的反向变化。在叶绿体结构变化的过程中，会合成和分解独特的蛋白质。为了分析油菜素内酯在光合作用中的作用，研究了14-C-表油菜素内酯在植物细胞中的胞间分布。叶绿体组分中放射性较高。其次，确定了14-C在叶绿体中的定位。类囊体组分的放射性以叶绿体组分最高，并随时间的推移而增加。水稻培养细胞的一个显著特征是淀粉粒的产生与类囊体体的形成同步。淀粉粒在叶绿体发育过程中保留下来，但在脱分化阶段消失。另一种是叶绿体脱分化形成的无叶绿体原生质体。

Interconversion of rice plastids was studies by electron microscopy and SDS-PAGE of plastid proteins. From electron microscopic observation, degreening process from chloroplasts to proplastids was a reverse change of greening from proplastids to chloroplasts. During the structural changes of plastids, distinctive proteins are synthesized and decomposed. It is suggested that these proteins are concernde in structural support and interconversion of plastids.To analysis a participation of brassinolide in photosynthesis, the intercellular distribution of 14-C-epibrassinolide in plant cells was investigated. The radioactivity was high in chloroplast fraction. Secondly, localization of 14-C in chloroplasts was determined. The radioactivity of thylakoid fraction was highest in plastid fraction and increased with the passage of time.A distinctive feature of plastid development in rice cultured cells i starch grain production synchronized with thylakoid formation. Starch grains remain through developmental process of plastids, but disappear in dedifferentiation stage. Another one is plastoglobule-less proplastids which resulted from chloroplast dedifferentiation.