Control of viral nervous necrosis in merine fish

浅海鱼病毒性神经坏死的控制

• 批准号: 05454094
• 负责人: NAKAI Toshihiro
• 金额: $ 2.94万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454094/
• 关键词: Viral disease; Viral nervous necrosis; Nodavirus; PCR; RNA; Coat protein; Marine fish; Antibody; 抗体検出

Viral nervous necrosis(VNN)is a newly emersing disease in hatchery-reared larvae and juveniles of marine fishes such as striped jack Pseudocaranx dentex in Japan. The causative agent of VNN in striped jack was identified as a nodavirus (Nodaviridae). Diseases of marine fishes similar to VNN were also reported in Southeast Asia, Australia, and Europe. In the case of VNN in striped jack, the brood stocks were the most important inoculum source of the virus, thus, elimination of spawners carrying the virus was thought to be effective to prevent the disease in larvae. Although removal of antibody-possessing spawners by ELISA-based diagnosis reduced the rate of disease occurrence, the disease still occurred in some larval groups obtained from antibody-negative spawners, suggesting that the absence of antibody did not necessarily mean the absence of the virus in spawners. In the next step, we applied the PCR amplification technique to detect the virus from spawner's gonad just before apawning and virus-positive spawners were eliminated from spawning groups. As a result, it was confirmed that this procedure was very effective for the prevention of VNN in the larval production.

病毒性神经坏死症（VNN）是一种发生于日本条纹鲷等海水鱼类孵化仔稚鱼的新疾病。经鉴定，条纹杰克VNN的病原为野田病毒（Nodaviridae）。在东南亚、澳大利亚和欧洲也有类似VNN的海洋鱼类疾病的报道。在条纹杰克VNN的情况下，亲鱼是病毒的最重要的接种源，因此，消除携带病毒的产卵被认为是有效地防止幼虫的疾病。虽然通过ELISA诊断去除具有抗体的产卵体降低了疾病发生率，但在从抗体阴性产卵体获得的一些幼虫组中仍然发生疾病，这表明抗体的缺乏并不一定意味着产卵体中没有病毒。下一步，我们应用PCR扩增技术在产卵前对产卵鱼性腺进行病毒检测，并将阳性产卵鱼从产卵组中剔除。结果证实，该方法对于预防幼虫生产中的VNN非常有效。

项目成果

虫明敬一: "シマアジのウイルス性神経壊死症:仔魚の発病に対する親魚の抗体価および産卵飼育方法の影響" 水産増殖. 41. 327-332 (1993)

Keiichi Mushiaki：“条纹竹荚鱼病毒性神经坏死：亲鱼抗体滴度和产卵饲养方法对幼鱼发病的影响”渔业研究 41. 327-332 (1993)。

Mushiake,K.: "Control of VNN in striped jack:Selection of spawners based on the detection of SJNNV gene by polymerase chain reaction(PCR)" Fish Pathology. 29. 177-182 (1994)

Mushiake,K.：“条纹杰克中 VNN 的控制：基于聚合酶链式反应 (PCR) 检测 SJNNV 基因的产卵鱼选择”《鱼类病理学》。

Munday,B.L.: "Antigenic relationship of the picorna-like virus of larval barramundi,Lates calcarifer Bloch to the nodavirus of larval striped jack,Pseudocaranx dentex(Bloch & Schneider)" Australian Veterinary Journal. 71. 384- (1994)

Munday，B.L.：“尖吻鲈幼虫、晚尖吻鲈 Bloch 的小角藻样病毒与条纹杰克幼虫、拟鲫鱼 (Bloch) 的诺达病毒的抗原关系

Nishizawa,T.: "Polymerase chain reaction(PCR)amplification of RNA of striped jack nervous necrosis virus(SJNNV)" Diseases of Aquatic Organisms. 18. 103-107 (1994)

Nishizawa,T.：“条纹杰克神经坏死病毒（SJNNV）RNA 的聚合酶链反应（PCR）扩增”水生生物疾病。

Mushiake, K.: "Viral nervous necrosis(VNN)of striped jack : Effects of plasma antibody level of spawners and apawning conditions on the occurrence of disease in their offsprings" Suisanzoshoku. 41. 327-332 (1993)

Mushiake, K.：“条纹杰克的病毒性神经坏死（VNN）：产卵者血浆抗体水平和产卵条件对其后代疾病发生的影响”Suisanzoshoku。