Role of multifunctional proteins in biogenesis, metabolism and regulation of the mitochondrial outer membrane

多功能蛋白在线粒体外膜生物发生、代谢和调节中的作用

• 批准号: 394024777
• 负责人: Professor Dr. Nikolaus Pfanner
• 金额: --
• 依托单位: Institut für Biochemie und Molekularbiologie
• 依托单位国家: 德国
• 项目类别: Reinhart Koselleck Projects
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/394024777?language=en
• 关键词: Role; multifunctional; proteins; biogenesis; metabolism

The mitochondrial outer membrane links mitochondria to other cellular compartments and performs crucial functions in metabolism, protein and lipid biogenesis, membrane dynamics, signaling and quality control. The outer membrane contains about 80 different proteins, raising the question how the numerous tasks can be performed and coordinated by this limited set of proteins. By analyzingthe network of outer membrane preprotein translocases, we observed an unexpectedly high number of proteins that perform more than one function. To test our hypothesis that multifunctional proteins play a central role, we will define a comprehensive protein interaction network of the mitochondrial outer membrane in the model organism baker’s yeast. Proteins interacting with atleast two different partners will be subjected to a molecular dissection to define distinct functions and to identify mechanisms for switching between functional states. We expect dual functional assignments at least in the following categories: protein translocation & assembly, metabolite transfer, lipid metabolism, membrane architecture, and regulatory processes. Our goal is tounderstand the molecular mechanisms and functional organization of the mitochondrial outer membrane protein network, thus establishing a paradigm for defining the importance of multifunctional proteins in an entire membrane system.

线粒体外膜将线粒体与其他细胞区室连接起来，并在代谢、蛋白质和脂质生物发生、膜动力学、信号传导和质量控制中发挥重要功能。外膜含有大约 80 种不同的蛋白质，这就提出了如何通过这组有限的蛋白质来执行和协调众多任务的问题。通过分析外膜前蛋白转位酶网络，我们观察到执行多种功能的蛋白质数量出人意料地多。为了检验我们的假设，即多功能蛋白质发挥核心作用，我们将定义模型生物面包酵母中线粒体外膜的全面蛋白质相互作用网络。与至少两个不同伙伴相互作用的蛋白质将进行分子解剖，以定义不同的功能并确定功能状态之间切换的机制。我们预计至少在以下类别中具有双重功能分配：蛋白质易位和组装、代谢物转移、脂质代谢、膜结构和调节过程。我们的目标是了解线粒体外膜蛋白网络的分子机制和功能组织，从而建立一个范式来定义多功能蛋白在整个膜系统中的重要性。