High temperature during grain filling induces transgenerational heat stress memory in rice (Oryza sativa L.)

灌浆期高温诱导水稻跨代热应激记忆（Oryza sativa L.）

• 批准号: 18J20784
• 负责人: SURIYASAK CHETPHILIN
• 金额: $ 1.41万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-04-25 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18J20784/
• 关键词: Heat stress; Rice; Grain filling; Epigenetics; Transgeneration; Flowering; Thermotolerance

Seeds developed under high temperature showed significantly delayed germination compared to control under the same non-stressed condition. This delay in germination was due to transcriptional changes after imbibition caused by hyper-methylation of ABA catabolism genes, and α-amylase gene. Higher methylation levels of target genes were confirmed by both Chop-qPCR and MeDIP-qPCR, which showed similar results stating that hyper-methylation of ABA catabolism genes and of α-amylase genes was involved in delayed seed germination of heat stressed seeds.At natural condition, plants grown from heat stressed seeds developed more tillers together with reduced height during vegetative growth despite unchanged total biomass compared to control plants. At harvest, heat stressed plants showed higher total panicle per plant, resulting in higher total spikelet number resulting higher yield. Other yield components, spikelet per panicle, 1000-grain weight, harvest index and seed setting rate, remained unchanged.We observed that early flowering phenotype and changes in leaf morphology were not cultivar-specific traits, by observing other 22 Japonica rice cultivars. Cultivar differences show that heat stress during grain filling induces transgenerational memory to the progeny to some content depending on cultivars.

在相同的非胁迫条件下，与对照相比，在高温下发育的种子表现出显着延迟发芽。这种发芽延迟是由于吸胀后阿坝催化剂基因和α-淀粉酶基因的超甲基化引起的转录变化。Chop-qPCR和MeDIP-qPCR均证实了目的基因的高甲基化，结果表明阿坝catalysts基因和α-淀粉酶基因的高甲基化与热胁迫种子延迟萌发有关。在收获时，热胁迫植株显示出较高的单株总穗数，导致较高的总小穗数，从而导致较高的产量。通过对22个粳稻品种的观察，发现早花表型和叶片形态的变化不是品种特异性的。品种间差异表明，灌浆期热胁迫诱导后代产生一定程度的跨代记忆。

项目成果

Heat stress during grain filling induces delayed seed germination in rice (Oryza sativa L.) through DNA methylation

灌浆期间的热应激通过 DNA 甲基化导致水稻 (Oryza sativa L.) 种子发芽延迟

• 发表时间: 2019
• 作者: Chetphilin Suriyasak;Nong Thi Hue;Takuya Kobayashi;Yui Oyama;Wun Jin Chen;Ryo Matsumoto;Norimitsu Hamaoka;Mari Iwaya-Inoue;Yushi Ishibashi
• 通讯作者: Yushi Ishibashi