Exploring natural analogues of future oceans to determine the adaptive potential of marine fishes to climate change

在本财政年度，我们与IRD(法国发展研究所)的研究人员合作进行了一次抽样旅行，从新喀里多尼亚博拉克的红树林泻湖收集鱼类。这一地点在退潮期间受到低pH的影响，这模拟了到本世纪末预测的OA条件。我们收集了5个物种，总共149条鱼。采集到的物种包括三种淡水鱼Dascyllus aruanus，Chromis viridis，Pomacentus moluccens，以及两种红衣鱼Zoramia letacantha和Zoramia viridiventer。从样本的大脑和鳃中提取了RNA并进行了测序，我们目前正在进行数据分析。我们还从在日本滋京的一个二氧化碳渗漏点及其周围收集的136条长尾鱼的鳍片中提取了DNA，以检查在渗漏点发现的鱼类的种群结构。在Shikine二氧化碳渗漏处收集的环境DNA(EDNA)的结果在澳大利亚和新西兰环境DNA会议上公布。2023年2月，塔斯马尼亚州霍巴特。

During this fiscal year we conducted a sampling trip in collaboration with the researchers from the IRD (Research Institute for Development, France) collected fishes from mangrove lagoon in Borake New Caledonoia. This site is subjected to low pH during the outgoing tide which simulate predicted conditions of OA by the end of the century. We collected five species for a total of 149 fishes. The species collected include three species of damselfish Dascyllus aruanus, Chromis viridis, Pomacentrus moluccensis, and two species of cardinal fish Zoramia leptacantha, and Zoramia viridiventer. RNA was extracted from the brain and gills of the samples and sequenced and we are currently working on the data analysis. We also extracted DNA from 136 fin clips of Pomacentrus coelestis collected in and around a CO2 seep in Shikine, Japan to examine the population structure of fishes found at the seep. Results from environmental DNA (eDNA) collected at the Shikine CO2 seep was presented at the Australia & New Zealand Environmental DNA Conference. Hobart, Tasmania February 2023.