Exploring natural analogues of future oceans to determine the adaptive potential of marine fishes to climate change

于本财政年度，我们与法国发展研究所（IRD）的研究人员合作进行了一次采样之旅，从Borake New Caledonoia的红树林泻湖采集鱼类。在退潮期间，该地点的pH值较低，模拟了世纪末OA的预测条件。共采集到5种鱼类149条。采集的物种包括三种雀鲷Dascyllus aruanus，Chromis viridis，Pomacentrus moluccensis，和两种主要鱼类Zoramia leptacantha和Zoramia viridiventer。从样本的大脑和鳃中提取RNA并进行测序，我们目前正在进行数据分析。我们还提取了DNA从136鳍夹的Pomacentrus coelestis收集和周围的一个CO2渗漏在日本的四金，研究在渗漏的鱼类种群结构。从志根CO2渗漏收集的环境DNA（eDNA）的结果在澳大利亚和新西兰环境DNA会议上发表。霍巴特，塔斯马尼亚2023年2月。

During this fiscal year we conducted a sampling trip in collaboration with the researchers from the IRD (Research Institute for Development, France) collected fishes from mangrove lagoon in Borake New Caledonoia. This site is subjected to low pH during the outgoing tide which simulate predicted conditions of OA by the end of the century. We collected five species for a total of 149 fishes. The species collected include three species of damselfish Dascyllus aruanus, Chromis viridis, Pomacentrus moluccensis, and two species of cardinal fish Zoramia leptacantha, and Zoramia viridiventer. RNA was extracted from the brain and gills of the samples and sequenced and we are currently working on the data analysis. We also extracted DNA from 136 fin clips of Pomacentrus coelestis collected in and around a CO2 seep in Shikine, Japan to examine the population structure of fishes found at the seep. Results from environmental DNA (eDNA) collected at the Shikine CO2 seep was presented at the Australia & New Zealand Environmental DNA Conference. Hobart, Tasmania February 2023.