The production of the pigs with an artificial gene (a functional domain-polymeriged nucleotide based on optimal codonusage)

使用人工基因（基于最佳密码子使用的功能域聚合核苷酸）生产猪

• 批准号: 23659612
• 负责人: MIYAGAWA Shuji
• 金额: $ 2.33万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23659612/
• 关键词: 異種移植; トランスジェニックブタ; ハイブリッド遺伝子; 顕微授精; 遺伝子構築; nsulin promoter; C1-INH; Thrombomodulin; DAF; MCP; 胚移植試験; 移植・再生医療; 応用動物; トランスレーションリサーチ; 動物; バイオテクノロジー

T o establish transgenic pigs with multiple human genes, the hybrid gene containing the function domains of each molecule were produced and the pigs with the gene was then tried to produce by intracytoplasmic sperm injection(ICSI) methods. At first, the transgenic mouse with the hybrid genes were successfully produced and analyzed. Concerning the pig project, the condition of the oocyte with each gene was studied under the microscope. The condition of the segmentation was no problem, however the pigs with genes were not born in this period.

为了建立多基因转基因猪，首先制备了含有每个分子功能域的杂合基因，然后利用卵胞浆内单精子注射（ICSI）方法制备了转基因猪。首先，成功地制备了具有杂交基因的转基因小鼠并进行了分析。关于猪项目，在显微镜下研究了具有每个基因的卵母细胞的情况。分割的条件是没有问题的，但是有基因的猪不是在这个时期出生的。

项目成果

Monocytic suppressor cells derived from peripheral blood suppress CTL lysis in xenotransplantation

外周血来源的单核细胞抑制细胞抑制异种移植中的 CTL 裂解

• 发表时间: 2012
• 作者: Nakayama Y;Kanda K;宮川 周士
• 通讯作者: 宮川 周士

The blocking of CCR5 and CXCR3 ・・・

CCR5 和 CXCR3 的阻断...

• 发表时间: 2012
• 期刊: Transplantation. In press
• 作者: Y.Kakuta;M.Okumi;S.Miyagawa; at all
• 通讯作者: at all

Trial using pig cells with the H–D antigen knocked down

• DOI: 10.1007/s00595-012-0274-x
• 发表时间: 2013-07
• 期刊: Surgery Today
• 影响因子: 2.5
• 作者: A. Yamamoto;K. Ikeda;Dandan Wang;S. Nakatsu;Y. Takama;T. Ueno;H. Nagashima;A. Kondo;M. Fukuzawa;S. Miyagawa

lectin microarray study of glycoantigens in neonatal porcine islet-like cell clusters (NPCC)

新生猪胰岛样细胞簇 (NPCC) 糖抗原的凝集素微阵列研究

• 发表时间: 2013
• 期刊: J Surgical Research
• 作者: Akira Maeda;Takehisa Ueno;Shino Nakatsu;Dandan Wang;Noriaki Usui;Shunsaku Takeishi;Teru Okitsu;Masafumi Goto;Hiroshi Nagashima;Shuji Miyagawa
• 通讯作者: Shuji Miyagawa

A study of the glycoantigens of neonatal ・・・

新生儿糖抗原的研究...

• 发表时间: 2012
• 期刊: Transplant Proc. In press.
• 作者: S.Nakatsu;Y.Takama;T. Ueno;C. Inuinaka,at all
• 通讯作者: C. Inuinaka,at all