Exploration of molecular mechanism underlying immune modulation by mast cells

在这项研究中，通过关注真皮肥大细胞（MC），我们试图确定与转移到淋巴结的功能变化，并了解MC的成熟和激活的分子机制，由前列腺素（PGs）驱动。我们鉴定了一类在转移到淋巴结后在特定T细胞亚群中上调的基因。我们发现未成熟MC释放的PLA 2G 3刺激成纤维细胞合成PGD 2，PGD 2通过DP 1受体刺激MC成熟。我们进一步发现，PGE 2通过MC激活以EP 3-Gi依赖的方式触发皮炎。

In this study, by focusing on dermal mast cells (MCs), we tried to identify functional changes in association with transition to lymph node, and to understand molecular mechanism underlying MC maturation and activation driven by prostaglandins (PGs). We identified a class of genes up-regulated in particular subset of T cells upon transition to lymph node. We found that PLA2G3 released from immature MC stimulates PGD2 synthesis in fibroblasts, and the PGD2 stimulates MC maturation through DP1 receptor. We further found that PGE2 triggers dermatitis by MC activation in an EP3-Gi-depepndent manner.