Analysis of neural plasticity and behavioral diversity by the inducible method of single neuron gene expression

单神经元基因表达诱导法分析神经可塑性和行为多样性

• 批准号: 24650165
• 负责人: TANIMOTO Masashi
• 金额: $ 2.5万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650165/
• 关键词: 遺伝子発現誘導; 神経細胞; 近赤外レーザー; 光ファイバ; 神経科学; 神経行動学; 神経可塑性; 赤外線レーザ; 熱ショック; IR-LEGO

Behavioral analysis on sound/vibration-evoked fast escapes in larval zebrafish showed that Mauthner (M-) cell played a crucial role in the initiation of the escapes. In the absence of the M-cell, non-M circuits could trigger the fast escapes to the left or right, while larvae sometimes exhibited aberrant escapes. By using transgenic lines that drive heat-inducible expression of green fluorescent protein (GFP), edge-processed optical fibers and infrared laser, we succeeded in inducing GFP in targeted single or multiple neurons in embryonic and larval zebrafish brain.

对斑马鱼幼体声/振快速逃逸的行为学分析表明，M-细胞在快速逃逸的启动过程中起着关键作用。在没有M细胞的情况下，非M环路可触发向左或向右的快速逃逸，而幼虫有时表现出异常逃逸。通过利用热诱导表达绿色荧光蛋白(GFP)的转基因品系、边缘处理光纤和红外激光，我们成功地在斑马鱼胚胎和仔鱼脑中的单个或多个靶向神经元中诱导了GFP。

项目成果

Functional diversity and similarity of morphologically homologous reticulospinal neurons in larval zebrafish hindbrain

斑马鱼幼虫后脑形态同源网状脊髓神经元的功能多样性和相似性

• 发表时间: 2012
• 作者: Aoba Mishiro;Masashi Tanimoto;Yoichi Oda
• 通讯作者: Yoichi Oda

Sound/vibration-evoked fast escapes triggered by Mauthner circuits or non-Mauthner circuits in zebrafish

斑马鱼中由莫特纳电路或非莫特纳电路触发的声音/振动诱发的快速逃逸

• 发表时间: 2013
• 作者: Tanimoto M;Yokomichi S;Sugimoto A;Oda Y
• 通讯作者: Oda Y

The large otolith and the transmission property in saccular pathway underlie auditory perception in larval zebrafish

大耳石和囊状通路的传输特性是斑马鱼幼虫听觉感知的基础

• 发表时间: 2012
• 作者: Maya Inoue;Masashi Tanimoto;Yoichi Oda
• 通讯作者: Yoichi Oda

Functional role of the Mauthner cell on the sound/vibration-evoked fast escapes in zebrafish

毛特纳细胞对斑马鱼声音/振动引起的快速逃逸的功能作用

• 发表时间: 2012
• 作者: Masashi Tanimoto;Sena Yokomichi;Yoichi Oda
• 通讯作者: Yoichi Oda

Mauthner cell activity associated with initiation and modification of fast escape behavior in zebrafish larva

毛特纳细胞活性与斑马鱼幼虫快速逃逸行为的启动和改变相关

• 发表时间: 2013
• 作者: Takahashi M;Tanimoto M;Oda Y
• 通讯作者: Oda Y