Development of the new cerebral infarction therapy by HSP27 protein

探讨重组活化HSP27蛋白的制备方法，为HSP27蛋白治疗新型脑梗死的临床应用奠定基础。从大肠杆菌中分离得到的去磷酸化重组HSP27的脑保护活性较低。重组HSP27蛋白被磷酸化。磷酸化重组HSP27具有脑保护作用。给予磷酸化重组HSP27后，脑卒中模型小鼠梗死体积减少52%。我们建立了体外批量生产活化磷酸化重组热休克蛋白27的方法。

We examined the method of producing activated recombinant HSP27 for future clinical application of the new cerebral infarction treatment by HSP27 protein. Dephosphorylated recombinant HSP27 which made and purified from Escherichia coli had low brain protection activity. The recombinant HSP27 protein was phosphorylated. Phosphorylated recombinant HSP27 had brain protection. Infarct volume was reduced by 52% in stroke model mice when phosphorylated recombinant HSP27 was administered. We developed the method of mass-producing activated phosphoryled recombinant HSP27 in vitro.