Development of model mouse series by dividing multi functions of transcriptional co-repressor

转录辅阻遏物多功能分化模型小鼠系列开发

• 批准号: 24650242
• 负责人: SHIROISHI TOSHIHIKO
• 金额: $ 2.5万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650242/
• 关键词: 代謝; モデルマウス; 遺伝学; ゲノム; 進化; 変異誘発

To understand the function of Hairless (Hr) in metabolism, we tried to develop a novel model mouse series in which Hr gene is conditionally targeted or over-expressed in a tissue-specific manner. Using Cre-loxP system, we developed conditional knockout mice of Hr gene, and CAG-loxP-CAT-pA-loxP-Hr transgenic mice that conditionally overexpress Hr gene. We also developed skeletal muscle specific Cre mice expressing Cre recombinase driven by Creatine kinase, muscle promoter. Using these genetically modified mice, we generated skeletal muscle specific Hr targeted

为了解无毛基因（Hairless，Hr）在代谢中的作用，我们尝试建立一种新的无毛小鼠模型，其中Hr基因以组织特异性方式被条件性靶向或过表达。利用Cre-loxP系统建立了Hr基因的条件性敲除小鼠和条件性过表达Hr基因的CAG-loxP-CAT-pA-loxP-Hr转基因小鼠。我们还开发了骨骼肌特异性Cre小鼠，其表达由肌启动子肌酸激酶驱动的Cre重组酶。使用这些转基因小鼠，我们产生了骨骼肌特异性Hr靶向

项目成果

エネルギー代謝制御におけるHairlessの新たな役割

Hairless在能量代谢控制中的新作用

• 发表时间: 2012
• 作者: 田中成和;高田豊行;城石俊彦
• 通讯作者: 城石俊彦