Research for development of biomarkers for radiation therapy and chemotherapy

放射治疗和化学治疗生物标志物开发研究

• 批准号: 24650640
• 负责人: MASUTANI Mitsuko
• 金额: $ 2.5万
• 依托单位: National Center of Neurology and Psychiatry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650640/
• 关键词: ポリ(ADP-リボース); PARP-1; 代謝物; リボシルアデノシン; リボシルイノシン; 血液; 尿; マウス

To identify a good biomarker for monitoring effectiveness of radiation therapy and chemotherapy, here we focused on the specific metabolites of poly(ADP-ribose) and evaluated their significance. We have established a measurement system for the metabolites of poly(ADP-ribose). The metabolism of poly(ADP-ribose) was analyzed in mouse bloodstream and urine. When poly(ADP-ribose) was intravenously injected into mice, poly(ADP-ribose) was rapidly degraded and the metabolites appeared in urine. We have further improved the pretreatment conditions of samples and optimized for LC-MS conditions for the measurement. After gamma-irradiation, the metabolites of poly(ADP-ribose) were detected in the mouse blood samples.

为了寻找一种良好的生物标志物来监测放疗和化疗的有效性，我们重点研究了聚（ADP-核糖）的特异性代谢产物，并评估了它们的意义。建立了聚ADP核糖代谢产物的检测系统。分析了聚（ADP-核糖）在小鼠血流和尿液中的代谢。当聚ADP-核糖静脉注射到小鼠体内时，聚ADP-核糖迅速降解，并在尿液中出现代谢产物。我们进一步改进了样品的前处理条件，优化了LC-MS测定条件。照射后，在小鼠血液样品中检测到聚（ADP-核糖）的代谢产物。

项目成果

Identification of ribosyladenosine and ribosylinosine as specific metabolites of poly(ADP-ribose)

鉴定核糖腺苷和核糖肌苷作为聚（ADP-核糖）的特定代谢物

• 发表时间: 2013
• 作者: Mitsuko Masutani;Hiroaki Fujimori;Tomoko Yoshida;Hiromi Harada;Motoharu Kohata;Takeji Takamura-Enya
• 通讯作者: Takeji Takamura-Enya

Rapid degradation of poly(ADP-ribose) after injection into the mouse bloodstream

注射到小鼠血液中后聚（ADP-核糖）快速降解

• 发表时间: 2013
• 期刊: Biol. Pharm. Bull.
• 作者: Yasuhisa Okajima;Tomoko Yoshida;Hiroaki Fujimori;Junhui Wang;Hiromi Harada;Ylenia Suzuki;Hisanori Suzuki;Mitsuko Masutani
• 通讯作者: Mitsuko Masutani

Rapid degradation of poly(ADP-ribose) after injection into the mouse bloodstream.

注射到小鼠血液中后聚（ADP-核糖）快速降解。

• 发表时间: 2013
• 期刊: Biol. Pharm. Bull.
• 作者: Okajima Y;Yoshida T;Fujimori H;Wang J;Harada H;Suzuki Y;Suzuki H;Masutani M.
• 通讯作者: Masutani M.

Identification of ribosyladenosine and ribosylinosine as specific metabolites of poly(ADP-ribose) in blood and urine.

鉴定核糖腺苷和核糖肌苷作为血液和尿液中聚（ADP-核糖）的特定代谢物。

• 发表时间: 2013
• 作者: Mitsuko Masutani;Hiroaki Fujimori;Tomoko Yoshida;Hiromi Harada;Motoharu Kohata;Takeji Takamura-Enya
• 通讯作者: Takeji Takamura-Enya