Research for development of biomarkers for radiation therapy and chemotherapy

为了寻找一种良好的生物标志物来监测放疗和化疗的有效性，我们重点研究了聚（ADP-核糖）的特异性代谢产物，并评估了它们的意义。建立了聚ADP核糖代谢产物的检测系统。分析了聚（ADP-核糖）在小鼠血流和尿液中的代谢。当聚ADP-核糖静脉注射到小鼠体内时，聚ADP-核糖迅速降解，并在尿液中出现代谢产物。我们进一步改进了样品的前处理条件，优化了LC-MS测定条件。照射后，在小鼠血液样品中检测到聚（ADP-核糖）的代谢产物。

To identify a good biomarker for monitoring effectiveness of radiation therapy and chemotherapy, here we focused on the specific metabolites of poly(ADP-ribose) and evaluated their significance. We have established a measurement system for the metabolites of poly(ADP-ribose). The metabolism of poly(ADP-ribose) was analyzed in mouse bloodstream and urine. When poly(ADP-ribose) was intravenously injected into mice, poly(ADP-ribose) was rapidly degraded and the metabolites appeared in urine. We have further improved the pretreatment conditions of samples and optimized for LC-MS conditions for the measurement. After gamma-irradiation, the metabolites of poly(ADP-ribose) were detected in the mouse blood samples.