Approaches to enhance a repair ability of UV induced DNA damage by promotion of DNA damage-recognition

环丁烷嘧啶二聚体(CPDS)是太阳紫外线引起的DNA损伤的主要类型之一，在人类中仅由核苷酸切除修复系统(NER)修复。NER的效率很大程度上取决于DNA损伤的螺旋扭曲。因此，增加CPDS的螺旋失真可能会提高NER的效率。我们克隆了抗CPDS抗体的VH和VL基因，构建了含有SV40缺失定位信号的单链抗体(ScFv)。单链抗体与CPDS的结合亲和力明显低于原免疫球蛋白。然后，我们构建了含有C-末端SV40NLS的Fab。它有效地与CPD结合。

Cyclobutane pyrimidine dimers (CPDs) is one of the major types of DNA damage induced by solar UV and is repaired exclusively by a nucleotide excision repair system (NER) in humans. The efficiency of NER mostly depends on helix-distortion of DNA lesion. Therefore, increasing the helix-distortion by CPDs may enhance NER efficiency. We cloned VH and VL genes of anti-CPDs antibody and constructed a single-chain Fv (ScFv) containing C-terminal SV40 nulear localization signal. The binding affinity of the scFv for CPDs was significantly lower than that of original IgG. Then we constructed a Fab containing C-terminal SV40 NLS. It effectively bound to CPDs.