Synthesis of Apatite Nanocrystals Containing Chlorophyll Molecules and the Antibody Immobilization towards Early Cancer Diagnosis

含叶绿素分子的磷灰石纳米晶的合成及抗体固定化用于早期癌症诊断

• 批准号: 24651133
• 负责人: TAGAYA MOTOHIRO
• 金额: $ 2.16万
• 依托单位: Nagaoka University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24651133/
• 关键词: 生体親和性材料; 細胞可視化技術; アパタイト; メソポーラスシリカ; クロロフィル; フルオレセイン; ナノバイオテクノロジー; 無機／有機ハイブリッド材料; 細胞可視化材料; 核形成; 希土類イオン; バイオイメージング; 診断; 発光; 希土類元素; 色素; 水酸アパタイト

The detection technique in which cancer cells at early stages can be specifically imaged by highly-biocompatible marker materials has been desired. In this study, biocompatible luminescent inorganic/organic hybrid nanoparticles were prepared in order to detect smaller tumor locations at the cellular sizes. As a result, nucleation reaction of inorganic apatite (or titania) effectively occurred on the end groups of highly-biocompatible dye molecules to successfully obtain the novel hybrid nanoparticles. The ligand molecules that can selectively react with cancer cells were chemically immobilized on the hybrid nanoparticles. The immobilized nanoparticles exhibited nontoxicity and specifically bound to cancer cells to resultantly induce intense orange (or green) luminescence. The hybrid nanoparticles that effectively work on cancer cells will provide novel concepts for non-invasive bioimaging as well as drug therapy for cancer diseases.

已经期望可以通过高度生物相容性的标记材料对早期阶段的癌细胞进行特异性成像的检测技术。在这项研究中，生物相容性发光无机/有机混合纳米粒子的制备，以检测在细胞大小的较小的肿瘤位置。结果，无机磷灰石（或二氧化钛）的成核反应有效地发生在高生物相容性染料分子的端基上，成功地获得了新型的杂化纳米颗粒。可以选择性地与癌细胞反应的配体分子被化学固定在杂化纳米颗粒上。固定化的纳米粒子表现出无毒性，并特异性地结合到癌细胞，从而诱导强烈的橙子（或绿色）发光。这种能有效作用于癌细胞的混合纳米粒子将为非侵入性生物成像以及癌症疾病的药物治疗提供新的概念。

项目成果

Control of Adhesion of Human Induced Pluripotent Stem Cells to Plasma-patterned Polydimethylsiloxane Coated with Vitronectin and gamma-globulin

人诱导多能干细胞对玻连蛋白和丙种球蛋白包被的等离子体图案化聚二甲基硅氧烷的粘附控制

• DOI: 10.1016/j.jbiosc.2014.02.009
• 发表时间: 2014
• 期刊: Journal of Bioscience and Bioengineering
• 影响因子: 2.8
• 作者: K. Shiba; M. Tagaya; N. Hanagata;Ryotaro Yamada; Koji Hattori; Saoko Tachikawa; Motohiro Tagaya (本研究代表者); Toru Sasaki; Shinji Sugiura; Toshiyuki Kanamori; Kiyoshi Ohnuma
• 通讯作者: Ryotaro Yamada; Koji Hattori; Saoko Tachikawa; Motohiro Tagaya (本研究代表者); Toru Sasaki; Shinji Sugiura; Toshiyuki Kanamori; Kiyoshi Ohnuma

Effect of Ultrasound on FT-IR Spectra of Poly (Acrylic Acid-co-N-Hydroxyethylacrylamide) in Aqueous Solution

超声波对水溶液中聚(丙烯酸-共-N-羟乙基丙烯酰胺) FT-IR 光谱的影响

• 发表时间: 2013
• 作者: S. Motozuka;M. Tagaya;T. Ikoma;T. Yoshioka;Z. Xu;J. Tanaka;Takaomi Kobayashi; Karla Lizette Tovar-Carrillo; Kazuki Nakasone; Motohiro Tagaya (本研究代表者);Ryotaro Yamada; Koji Hattori; Motohiro Tagaya (本研究代表者); Toru Sasaki; Shinji Sugiura; Toshiyuki Kanamori; Kiyoshi Ohnuma;Takaomi Kobayashi; Josue Addiel Venegas-Sanchez; Kohsuke Hirano; Motohiro Tagaya (本研究代表者);Kohtaroh Nakamoto; Ayaya Mikazuki; Masaru Ohshiro; Motohiro Tagaya (本研究代表者); Takaomi Kobayashi;Josue Addiel Venegas-Sanchez; Motohiro Tagaya (本研究代表者); Takaomi Kobayashi
• 通讯作者: Josue Addiel Venegas-Sanchez; Motohiro Tagaya (本研究代表者); Takaomi Kobayashi

High Frequency Sono-Treatment on Regeneration of Agave Tequilana Waste Fibers for Heavy Metal Ion Adsorbents

高频声处理再生龙舌兰龙舌兰废纤维用于重金属离子吸附剂

• 发表时间: 2013
• 作者: S. Motozuka;M. Tagaya;T. Ikoma;T. Yoshioka;Z. Xu;J. Tanaka;Takaomi Kobayashi; Karla Lizette Tovar-Carrillo; Kazuki Nakasone; Motohiro Tagaya (本研究代表者);Ryotaro Yamada; Koji Hattori; Motohiro Tagaya (本研究代表者); Toru Sasaki; Shinji Sugiura; Toshiyuki Kanamori; Kiyoshi Ohnuma;Takaomi Kobayashi; Josue Addiel Venegas-Sanchez; Kohsuke Hirano; Motohiro Tagaya (本研究代表者);Kohtaroh Nakamoto; Ayaya Mikazuki; Masaru Ohshiro; Motohiro Tagaya (本研究代表者); Takaomi Kobayashi;Josue Addiel Venegas-Sanchez; Motohiro Tagaya (本研究代表者); Takaomi Kobayashi;Karla Lizette Tovar-Carrillo; Motohiro Tagaya (本研究代表者); Takaomi Kobayashi;Kohsuke Hirano; Motohiro Tagaya (本研究代表者); Satoshi Sugita Sueyoshi; Takaomi Kobayashi
• 通讯作者: Kohsuke Hirano; Motohiro Tagaya (本研究代表者); Satoshi Sugita Sueyoshi; Takaomi Kobayashi

キノリンとアパタイトのハイブリッドナノ結晶の固相反応創出と界面光機能の発現

喹啉和磷灰石杂化纳米晶中固态反应的产生和界面光学功能的表达

• 发表时间: 2012
• 作者: 本塚智;多賀谷基博;生駒俊之;吉岡朋彦;許哲峰;田中順三
• 通讯作者: 田中順三

蛍光多孔質シリカナノ粒子の葉酸分子修飾と細胞可視化

荧光多孔二氧化硅纳米粒子的叶酸分子修饰及细胞可视化

• 发表时间: 2012
• 作者: 福持 裕之;松井 広志;田所 誠;多賀谷基博,生駒俊之,吉岡朋彦,許哲峰,本塚智,田中順三
• 通讯作者: 多賀谷基博,生駒俊之,吉岡朋彦,許哲峰,本塚智,田中順三