Development of fluorogenic probe for gene-analisis utilizing novel sililated fluorescent material

开发了具有可修饰的甲硅烷基化官能团的新型芘衍生物。其中，具有叠氮化物功能的衍生物通过点击化学成功地掺入具有炔基功能的修饰DNA中。首次合成了具有强吸电子基团（氰基）的硅烷基化芘。与未改性芘相比，衍生物的吸收光谱和发射光谱均发生了明显的红移。此外，衍生物的荧光量子产率增加了近两倍的未修饰的芘。这种独特的衍生物进一步衍生为相应的磷酰胺衍生物，并掺入到寡聚DNA的5 '端。

Novel pyrene derivatives having modifiable silylated functional group were developed. Among these, a derivative possessing azide function was successfully incorporated into a modified DNA having alkynyl function via click chemistry. Also, a silylated pyrene possessing strong electron-withdrawing group (cyano function) was synthesized first time. The derivative exhibited marked red-shift on both absorption and emission spectra compare to that of the unmodified pyrene. In addition, fluorescent quantum yield of the derivative was increased almost twice as much as that of the unmodified pyrene. This unique derivative was further derived to the corresponding phosphoramide derivative and was incorporated into 5'-terminus of oligoDNA.