Development of fluorogenic probe for gene-analisis utilizing novel sililated fluorescent material

利用新型硅化荧光材料开发用于基因分析的荧光探针

• 批准号: 24651255
• 负责人: SHINOZUKA Kazuo
• 金额: $ 2.16万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24651255/
• 关键词: バイオプローブ; 含ケイ素高輝度蛍光剤; 蛍光核酸プローブ; シリル化ピレン; 高輝度蛍光剤

Novel pyrene derivatives having modifiable silylated functional group were developed. Among these, a derivative possessing azide function was successfully incorporated into a modified DNA having alkynyl function via click chemistry. Also, a silylated pyrene possessing strong electron-withdrawing group (cyano function) was synthesized first time. The derivative exhibited marked red-shift on both absorption and emission spectra compare to that of the unmodified pyrene. In addition, fluorescent quantum yield of the derivative was increased almost twice as much as that of the unmodified pyrene. This unique derivative was further derived to the corresponding phosphoramide derivative and was incorporated into 5'-terminus of oligoDNA.

开发了具有可改性硅化官能团的新型芘衍生物。其中，一个具有叠氮化物功能的衍生物通过点击化学被成功地结合到一个具有炔基功能的修饰DNA中。并首次合成了具有强吸电子基团（氰基）的硅基化芘。与未改性的芘相比，该衍生物在吸收光谱和发射光谱上都表现出明显的红移。此外，该衍生物的荧光量子产率比未修饰的芘提高了近两倍。这个独特的衍生物被进一步衍生为相应的磷酰胺衍生物，并被整合到oligoDNA的5'端。

项目成果

Development of Quencher-free Dumbbell-form Molecular Beacon Probe Having Silylated Pyrene

具有硅烷化芘的无猝灭剂哑铃型分子信标探针的研制

• 发表时间: 2013
• 作者: T. Moriguchi;N. Takayama;S. Watanabe;N. Kanazawa;K. Shinozuka
• 通讯作者: K. Shinozuka

Elemet Inovation 研究活動概要

Elemet Innovation 研究活动概述

Development of Novel Push-Pull Type Silylated Pyrene Derivatives as DNA Labeling Agents

新型推拉型硅烷化芘衍生物作为 DNA 标记剂的开发

• 发表时间: 2013
• 作者: S. Hida;T. Moriguchi;and K. Shinozuka
• 通讯作者: and K. Shinozuka

Development of Quencher-Free Dumbbell-Form Molecular Beacon Probe Having the Silylated Pyrene

具有硅烷化芘的无猝灭剂哑铃型分子信标探针的研制

• 发表时间: 2013
• 作者: T. Moriguchi;N. Takayama;S. Watanabe;N. Kanazawa;and K. Shinozuka
• 通讯作者: and K. Shinozuka

Pyrene: Chemical Properties, Biochemistry Applications and Toxic Effect

芘：化学性质、生物化学应用和毒性作用

• 发表时间: 2013
• 作者: K. Shinozuka;T. Takeuchi
• 通讯作者: T. Takeuchi