Development of fluorogenic probe for gene-analisis utilizing novel sililated fluorescent material

开发了具有可改性硅化官能团的新型芘衍生物。其中，一个具有叠氮化物功能的衍生物通过点击化学被成功地结合到一个具有炔基功能的修饰DNA中。并首次合成了具有强吸电子基团（氰基）的硅基化芘。与未改性的芘相比，该衍生物在吸收光谱和发射光谱上都表现出明显的红移。此外，该衍生物的荧光量子产率比未修饰的芘提高了近两倍。这个独特的衍生物被进一步衍生为相应的磷酰胺衍生物，并被整合到oligoDNA的5'端。

Novel pyrene derivatives having modifiable silylated functional group were developed. Among these, a derivative possessing azide function was successfully incorporated into a modified DNA having alkynyl function via click chemistry. Also, a silylated pyrene possessing strong electron-withdrawing group (cyano function) was synthesized first time. The derivative exhibited marked red-shift on both absorption and emission spectra compare to that of the unmodified pyrene. In addition, fluorescent quantum yield of the derivative was increased almost twice as much as that of the unmodified pyrene. This unique derivative was further derived to the corresponding phosphoramide derivative and was incorporated into 5'-terminus of oligoDNA.