Development of visualization technique of cancer cell by click chemistry

点击化学癌细胞可视化技术的开发

• 批准号: 24651264
• 负责人: XU YAN
• 金额: $ 2.58万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24651264/
• 关键词: 生体分子; 可視化; バイオテクノロジー; 核酸; 糖

The research project focuses on the development of visualization technique of cancer cell. Utilizing the high resolution of light on spatial and temporal, the fluorescent probe that synthesized on the basis of organic chemistry is used to visualize cancer cells. To light up the cancer cells by small organic probe molecules with specific and efficient click reaction on cancer cells, cancerous tumor can be detected in real time. We synthesized the nucleic acid and sugar derivatives having alkyne as a tracer. We further developed a coumarin molecule having an azide group as a profluorophore. The profluorophore coumarin has no fluorescence, whereas click reaction will result in a strong fluorescence. We then used the profluorophore moiety which serve as a light-up reporter to painting chromosome DNA based on click reaction and visualized the clear chromosomes in multicolor. The method has been successfully applied to detect the signaling molecule derivative 8-azido cAMP in cells.

该研究项目着重于癌细胞可视化技术的发展。利用光的高分辨率在空间和时间上，基于有机化学合成的荧光探针用于可视化癌细胞。为了通过针对癌细胞的特定有效的点击反应来点亮癌细胞，可以实时检测到癌性肿瘤。我们合成具有碱为示踪剂的核酸和糖衍生物。我们进一步开发了一个叠氮化物组作为大量浮动物的香豆素分子。大量香豆素没有荧光，而点击反应会导致强烈的荧光。然后，我们使用了大量的部分部分，该部分是一名基于点击反应的染色体DNA绘制染色体DNA的点燃记者，并在多色中可视化了透明的染色体。该方法已成功地用于检测细胞中的信号分子衍生物8-齐达营。

项目成果

宮崎大学医学部機能制御学講座物質科学分野研究室

宫崎大学医学院功能控制系材料科学实验室

DNA Conjugates and Sensors

DNA 缀合物和传感器

• 发表时间: 2012
• 作者: Kashida;H.; Asanuma;H.
• 通讯作者: H.

To connect cell by DNA forming G-quadruplex

通过DNA连接细胞形成G-四联体

• 发表时间: 2014
• 作者: Hongshan LIU;Takumi ISHIZUKA;Yan XU
• 通讯作者: Yan XU

Oligonucleotide Models of Telomeric DNA and RNA Form a Hybrid G-quadruplex Structure as a Potential Component of Telomeres

• DOI: 10.1074/jbc.m112.342030
• 发表时间: 2012-12-07
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Xu, Yan;Ishizuka, Takumi;Hayashi, Tetsuya
• 通讯作者: Hayashi, Tetsuya

Chromosome painting by using click chemistry : A light-up approach

使用点击化学进行染色体绘制：一种发光方法

• 发表时间: 2013
• 作者: Takumi Ishizuka;Yan Xu
• 通讯作者: Yan Xu