Development of site-specific conversion method of nucleobase in mRNA

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In this study, we aimed at the development of the method for conversion ofcytosine nucleobase into uracil one in messenger RNA. In order to implement the method, sulnic acid-conjugated oligonucleotides were designed as a new class of modified oligonucleotides, and their syntheses were achieved. Although the conversion reaction of cytosine nucleobase within 2'-O-methyl-RNA using the synthesized oligonucleotides under various conditions was investigated during this research period, we could not succeed in the development of the conversion method. However, this reseach provided useful information for the development of the method in future.