Development of selective cell collection method using photo-degradable PEG-lipid

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Single-cell array is a promising technology for microscopy-based high-content analyses.In this study, we constructed a versatile single cell array for both non-adherent and adherent cells by developing a photo-cleavable PEG-lipid/collagen coating material. On this material surface, we confirmed that cells behave similar to their native functions such as cell adhesion, mitosis and migration. Furthermore, we succeeded in high-throughput single cell quantitative imaging analysis on the motility of the adherent cells and that on the morphological changes of non-adherent cells using this PEG-lipid/collagen-based single-cell array, and the recovery of a target cell by photo-irradiation of this cell.This versatile single-cell array shall be invaluable tool for the research in the fields of cell engineering.